Veterinary Archives, Vol. 75 No. 3, 2005.
Original scientific paper
Concentrations of total proteins and albumins, and AST, ALT and GGT activities in the blood plasma of mares during pregnancy and early lactation
Suzana Milinković-Tur
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine,
Vedrana Perić
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine,
Zvonko Stojević
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine,
Maja Zdelar-Tuk
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine,
Jasna Piršljin
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine,
Abstract
The metabolism of proteins in the blood plasma of Holstein breed mares was investigated during pregnancy and early lactation. Blood for analysis was taken in the first, second and third periods of pregnancy, and during early lactation. Blood samples were collected by jugular puncture with heparin as an anticoagulant. In the obtained blood plasma the concentration of total proteins and albumin, and the activity of enzymes aspartate aminotransferase (AST; EC 2.6.1.1), alanine aminotransferase (ALT; EC 2.6.1.2), and gamma-glutamyltransferase (GGT; EC 2.3.2.2) was determined by spectrophotometry. According to the research results of the blood plasma in mares during pregnancy and early lactation, there were no significant differences in the concentration of total proteins, albumin, and ALT and GGT enzyme activity. In the final third of pregnancy there was a statistically significant decrease of AST activity compared to the first period (P<0.01), and the second period of pregnancy (P<0.001). The lower activity of AST during pregnancy and early lactation can be attributed to this special breed. The obtained results constitute a contribution to a better understanding of biochemical processes in pregnant mares, for estimating their physiological status and for diagnostic purposes.
Keywords
mares; pregnancy; early lactation; total plasma proteins; albumins; transferase
Hrčak ID:
37431
URI
Publication date:
20.6.2005.
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