Original scientific paper

Usporedna analiza sekvenci dijagnostičkih PCR umnožaka virusa ovčjih boginja iz indijske ovce

M. Parthiban ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
R. Govindarajan ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
S. Manoharan ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
V. Purushothaman ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
N. Daniel Joy Chandran ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India
A. Koteeswaran ; Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, Tamil Nadu, India

Abstract

Virus ovčjih boginja dokazan je u terenskim uzorcima imunodifuzijom u gelu i lančanom reakcijom polimeraze (PCR). PCR umnošci gena za protein ovojnice (192 pb) indijskih izolata virusa ovčjih boginja izdvojenih tijekom 1997. i 2003. razlikovali su se po nukleotidnom slijedu. Izolati iz 2003. međusobno su bili identični u 100% nukleotida, dok je izolat iz 1997. bio njima podudaran samo u 95% nukleotida i aminokiselina. Ti indijski izolati bili su jedinstveni u odnosu na druge viruse boginja koza po jednom ugrađenom kodonu. U skladu s prijašnjim istraživanjima, nalazi ukazuju da su nedavni izolati virusa ovčjih boginja u Indiji sličniji ranije opisanim virusima ovčjih boginja nego virusima kozjih boginja iz Indije i drugdje.

Keywords

virus ovčjih boginja; Indija; nukleotidni slijed; protein ovojnice; filogenetska analiza

Hrčak ID:

37432

URI

https://hrcak.srce.hr/37432

Publication date:

20.6.2005.

Article data in other languages: english

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