Original scientific paper

Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative

Christoph Reichert ; Rheinische Friedrich-Wilhelms-University, Department of Orthodontics, Germany
Marcus Klein ; Johannes Gutenberg-University Mainz, Department of Oral and Maxillofacial Surgery, Germany
Adrian Kasaj ; Johannes Gutenberg-University Mainz, Department of Operative Dentistry and Periodontology, Germany
Sebastian Kuhn ; Johannes Gutenberg University, Department of Trauma Surgery, Germany
Hermann Götz ; Johannes Gutenberg-University Mainz, Institute of Applied Structure- and Microanalysis, Germany
Gerhard Hommel ; Johannes Gutenberg-University Mainz, Institute of Medical Biostatistics, Epidemiology and Informatics, Germany
Heinz Duschner ; Johannes Gutenberg-University Mainz, Institute of Applied Structure- and Microanalysis, Germany
Bilal Al-Nawas ; Johannes Gutenberg-University Mainz, Department of Oral and Maxillofacial Surgery, Germany

Abstract

The precondition for successful periodontal regeneration is adequate activation of relevant cell populations like osteogenic cells. Here, cell adhesion and maturation are closely associated with cell morphology and f-actin cytoskeletal organisation. The potential of solitaire extracellular matrix (ECM) components as well as enamel matrix derivative (EMD) to enhance periodontal healing is well documented. Objective: The aim of the study was to test the impact of the ECM proteins collagen type 1 and laminin-1 as well as commercially available EMD on osteogenic cell morphology and cytoskeletal organisation. Material and methods: In an observational study, a total of 2450 osteogenic cells of 5 different cell lines (4 primary ones and 1 commercial one) cultivated on the respective substrates were analysed by 3 independent observers. After staining for the f-actin cytoskeleton and automated CLSM visualisation, cells were assigned to 3 different categories depending on morphological cell attributes (immature vs. intermediate vs. mature). Besides descriptive analysis, a multivariate logistic regression was performed to identify relevant influence parameters on cell morphology and cytoskeletal organisation. Results: The applied solitaire ligands collagen and laminin and especially EMD promoted a mature osteogenic phenotype. Nevertheless, considerable differences between the investigated cell lines could be identified as well. Analysis of cell morphology and cytoskeletal organisation offers a reliable method of acquiring the first hints of biocompatibility and bio-activation on different substrates. Conclusion: Our results highlight the potential of the investigated ligands to support periodontal regeneration by enhancing osteogenic cell attachment and maturation.

Keywords

Osteoblasts; Extracellular Matrix; Enamel Matrix Proteins; Cytoskeleton; Periodontal Diseases

Hrčak ID:

40777

URI

https://hrcak.srce.hr/40777

Publication date:

15.9.2009.

Article data in other languages: croatian

Visits: 2.785 *