Biochemia Medica, Vol. 19 No. 3, 2009.
Original scientific paper
5'-nucleotidase, oxidative stress and antioxidant status in alcohol consumers and cirrhotic patients
Tarannum F. Subhani
; Department of Biochemistry, Kasturba Medical College, Manipal University, Mangalore, India
Mohammad A. Nasar
; Department of Biochemistry, Faculty of Medicine, Al-Arab Medical University, Benghazi, Libya
Abdalla Jarrari
; Department of Biochemistry, Faculty of Medicine, Al-Arab Medical University, Benghazi, Libya
Vivian D’Souza
; Department of Biochemistry, Kasturba Medical College, Manipal University, Mangalore, India
Mohammad A. Naseer
; Department of Pharmaceutical Chemistry, Jamia Hamdard, New Delhi, India
Faiyaz Shakeel
; Department of Pharmaceutics, Faculty of Pharmacy, Al-Arab Medical University, Benghazi, Libya
Abstract
Background: The present study was undertaken to determine the 5'-nucleotidase enzyme activity in liver cirrhotic patients and alcohol consumers. Oxidative stress, antioxidants and their association with 5'-nucleotidase were also investigated.
Methods: The study included three groups of 25 age and sex matched subjects: group I (control), group II (alcohol consumers) and group III (cirrhotic patients). Blood samples were collected and centrifuged for separation of plasma for analysis of 5'-nucleotidase. Separated cells were washed thrice with 0.9% w/v cold normal saline and used for the analysis of glutathione, malondialdehyde and superoxide dismutase.
Results: The activity of serum 5'-nucleotidase was significantly increased in both cirrhotic patients and alcohol consumers. The levels of malondialdehyde were also significantly increased in both cirrhotic patients and alcohol consumers. The levels of glutathione and superoxide dismutase were significantly decreased in both cirrhotic patients and alcohol consumers.
Conclusions: Study results indicated the activity of serum 5'-nucleotidase to be consistently higher in cirrhotic patients and alcohol consumers. The difference recorded might be pointing to the extent of liver damage, hepatobiliary damage, and biliary stasis.
Keywords
alcohol consumers; antioxidant effects; 5'-nucleotidase; oxidative stress
Hrčak ID:
40857
URI
Publication date:
24.9.2009.
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