Original scientific paper
https://doi.org/10.2478/10004-1254-60-2009-1971
Inflammatory and Haematotoxic Potential of Indoor Stachybotrys chartarum (Ehrenb.) Hughes Metabolites
Elena Piecková
; Slovak Medical University, Bratislava, Slovakia
Marta Hurbánková
; Slovak Medical University, Bratislava, Slovakia
Silvia Černá
; Slovak Medical University, Bratislava, Slovakia
Aurélia Lišková
; Slovak Medical University, Bratislava, Slovakia
Zuzana Kováčiková
; Slovak Medical University, Bratislava, Slovakia
Zuzana Kolláriková
; Slovak Medical University, Bratislava, Slovakia
Soňa Wimmerová
; Slovak Medical University, Bratislava, Slovakia
Abstract
Mould Stachybotrys chartarum (Ehrenb.) Hughes is known to pose a health risk in indoor environments. Most of its strains can produce several intra- and extracellular trichothecene mycotoxins. Complex secondary metabolites of stachybotrys isolates from mouldy dwellings/public buildings in Slovakia were intratracheally instilled in Wistar male rats (4 μg in 0.2 mL of 0.2 % dimethylsulphoxide; diacetoxyscirpenol as the positive control). After three days, haematological parameters were measured in peripheral blood
and infl ammatory response biomarkers in bronchoalveolar lavage fl uid (BALF), and the results were statistically analysed. Exometabolites proved to suppress red blood cell (RBC), decreasing the total RBC count, haemoglobin, and haematocrit. The exposed rats showed signifi cantly higher total BALF cell count, indicating infl ammation, lower alveolar macrophage counts, and increased granulocyte count related to the BALF cells. Due to haematotoxic and infl ammation-inducing properties, metabolites of S. chartarum can
cause damage to the airways and haematological disorders in occupants of mouldy buildings.
Keywords
infl ammation; intratracheal instillation; moulds; rats; red blood cells
Hrčak ID:
45372
URI
Publication date:
22.12.2009.
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