Croatica Chemica Acta, Vol. 82 No. 4, 2009.
Original scientific paper
Simultaneous Kinetic Spectrophotometric Determination of Hydrazine and Isoniazid Using H-Point Standard Addition Method and Partial Least Squares Regression in Micellar Media
Mohammad Ali Karimi
; Department of Chemistry & Nanoscience and Nanotechnology Research Laboratory (NNRL), Faculty of Sciences, Payame Noor University (PNU), Sirjan, Iran; Department of Chemistry, Faculty of Sciences, Payame Noor University (PNU), Ardakan, Iran
Mohammad Mazloum-Ardakani
Mohammad Hossein Mashhadizadeh
Fatemeh Banifatemeh
; Department of Chemistry, Faculty of Sciences, Payame Noor University (PNU), Ardakan, Iran
Abstract
The present study describes the application of simultaneous kinetic spectrophotometric determination of hydrazine (HZ) and isoniazid (INH), using H-point standard addition method (HPSAM) and partial least squares (PLS) calibration. The methods are based on the difference observed in the rate of iron (III) reduction with HZ and INH, in the presence of 2,2'-bipyridine (Bpy) and the subsequent complex formation between the resulted Fe2+ and Bpy in a solution containing sodium dodecyl sulfate (SDS) as a micellar medium. INH and HZ can simultaneously be determined between the range of 0.08−6.0 and 1.0−80.0 μg mL−1, respectively. The results have shown that by the application of HPSAM, the simultaneous determination could be performed with the ratio of 1:1000 to 1:12.5 for INH-HZ. Through the HPSAM analysis, the relative standard deviations of HZ and INH were 2.5 and 1.2, respectively. The total relative standard error for applying the PLS method to 9 synthetic samples, in the concentration ranges of 0.0−20.0 μg mL−1 of HZ and 0.5−3.0 μg mL−1 of INH, was 3.19. Both proposed methods (PLS and HPSAM) were successfully applied to the simultaneous determination of HZ and INH in several commercially available isoniazid formulations and satisfactory results were obtained.
Keywords
isoniazid; hydrazine; simultaneous determination; HPSAM; PLS
Hrčak ID:
45534
URI
Publication date:
20.12.2009.
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