Original scientific paper

Age-related antioxidant enzyme activities and lipid peroxidation in heart muscles of broiler chickens fed with supplementary organic selenium

Suzana Milinković-Tur ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Jasna Aladrović ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Blanka Beer Ljubić ; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Nina Poljičak-Milas ; Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

The effect of organic selenium feed supplements on the activities of antioxidant enzymes and on lipid peroxidation was investigated in chickens’ heart muscles during growth. The experiment was carried out on Ross 308 broiler chickens of both sexes, either on standard diet (control) or receiving organic selenium supplementation in the standard diet (experimental). After two, four and six weeks of growth, ten chickens from each group were sacrificed by decapitation and heart muscles were collected for analyses. In the heart muscle the activities of glutathione peroxidase (GSH-Px), catalase (CAT), cooper zinc superoxide dismutase (Cu,Zn- SOD), manganese superoxide dismutase (Mn-SOD), reduced glutathione (GSH) and lipid peroxide (TBARS) were determined. During the experiments, age-related differences of antioxidant enzyme activities and GSH and TBARS concentrations were obtained in control and in experimental chicken. At the end of growth, after the 6th week of age, a significant increase of GSH-Px, Cu,Zn-SOD, and CAT activities (P<0.001; P<0.001; P<0.05)
with a significant decrease of Mn-SOD activity and GSH concentration (P<0.01; P<0.01) were obtained in the heart muscle of the experimental chickens. In the control chickens, signifi cant increases of GSH-Px, Cu,Zn- SOD and TBARS (P<0.001; P<0.001; P<0.01) with a significant decrease of GSH concentration (P<0.05) were obtained in the heart muscle after six weeks of age. After the fourth week of age, chickens with organic selenium supplementation had a significantly higher activity of GSH-Px, Cu,Zn-SOD, and Mn-SOD (P<0.001; P<0.05; P<0.05) than the control chickens of the same age. In conclusion, chickens with organic selenium supplementation in the diet maintained their antioxidant systems in the heart muscle more effectively, with enhanced antioxidant defence.

Keywords

chickens; organic selenium; heart muscle; antioxidant enzymes; lipid peroxidation

Hrčak ID:

45608

URI

https://hrcak.srce.hr/45608

Publication date:

26.10.2009.

Article data in other languages: croatian

Visits: 2.012 *