Original scientific paper

https://doi.org/10.2478/v10007-010-0017-8

Simultaneous spectrophotometric determination of losartan potassium, amlodipine besilate and hydrochlorothiazide in pharmaceuticals by chemometric methods

D. Nagavalli ; Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Kanchipuram District, Tamil Nadu, India
V. Vaidhyalingam ; Madras Medical College, Chennai-600003, India
A. Santha ; C. L. Baid Metha College of Pharmacy, Chennai-600 096. Tamil Nadu, India
A. S. K. Sankar ; Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Kanchipuram District, Tamil Nadu, India
O. Divya ; IIT, Chennai-600 036, Tamil Nadu, India

Abstract

In the present work, four different spectrophotometric methods for simultaneous estimation of losartan potassium, amlodipine besilate and hydrochlorothiazide in raw materials and in formulations are described. Overlapped data was quantitatively resolved by using chemometric methods, classical least squares (CLS), multiple linear regression (MLR), principal component regression (PCR) and partial least squares (PLS). Calibrations were constructed using the absorption data matrix corresponding to the concentration data matrix, with measurements in the range of 230.5350.4 nm (∆λ = 0.1 nm) in their zero order spectra. The linearity range was found to be 840, 15 and 315 μg ml1 for losartan potassium, amlodipine besilate and hydrochlorothiazide, respectively. The validity of the proposed methods was successfully assessed for analyses of drugs in the various prepared physical mixtures and in tablet formulations.

Keywords

losartan; amlodipine besilate; hydrochlorothiazide; spectrophotometry; chemometry

Hrčak ID:

48383

URI

https://hrcak.srce.hr/48383

Publication date:

1.6.2010.

Article data in other languages: croatian

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