Short communication, Note

https://doi.org/10.2478/v10007-010-0015-x

Use of N,N-diethyl-p-phenylenediamine sulphate for the spectrophotometric determination of some phenolic and amine drugs

Padmarajaiah Nagaraja ; Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore-570006, India
Ashwinee Kumar Shrestha ; Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore-570006, India
Anantharaman Shivakumar ; Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore-570006, India
Avinash K. Gowda ; Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore-570006, India

Abstract

Spectrophotometric methods are proposed for the determination of drugs containing a phenol group salbutamol sulphate (SLB), ritodrine hydrochloride (RTD), isoxsuprine hydrochloride (IXP) and drugs containing an aromatic amine group dapsone hydrochloride (DAP), sulfamethoxazole (SFM) and sulfadiazine (SFD) in pharmaceutical dosage forms. The methods are based on coupling of N,N-diethyl-p-phenylenediamine sulphate with the drugs in the presence of KIO4 to give a green colored product (λmax at 670 nm) and a red colored product (λmax at 550 nm), respectively. Linear relationships with good correlation coefficients (0.99860.9996) were found between absorbance and the corresponding concentration of drugs in the range 17, 222, 117, 1.512, 225, and 221 μg mL1 for SLB, RTD, IXP, DAP, SFM and SFD, respectively. Variable parameters such as temperature, reaction time and concentration of the reactants were analyzed and optimized. The RSD of intra-day and inter-day studies was in the range of 0.21.0 and 0.41.0 %, respectively. No interference was observed from common pharmaceutical adjuvants. The reliability and performance of the proposed methods was validated statistically; percentage recovery ranged from 99.5 ± 0.1 to 99.9 ± 0.3 %. Limits of detection were 0.14, 0.21, 0.51, 0.44, 0.33 and 0.37 µg mL1 for SLB, RTD, IXP, DAP, SFM, and SFD, respectively.

Keywords

N,N-diethyl-p-phenylenediamine sulphate; UV/Vis spectrophotometry; oxidation; coupling reaction

Hrčak ID:

48385

URI

https://hrcak.srce.hr/48385

Publication date:

1.6.2010.

Article data in other languages: croatian

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