Short communication, Note

https://doi.org/10.2478/10004-1254-61-2010-1985

Qualitative GC-MS Assessment of TCP and TAMORF Elimination in Rats

Maja Jelena Petek orcid.org/0000-0002-3659-1725 ; Forensic Science Centre “Ivan Vučetić”, Zagreb, Croatia
Ana Lucić Vrdoljak ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Gordan Mršić ; Forensic Science Centre “Ivan Vučetić”, Zagreb, Croatia

Abstract

Nerve agents are highly toxic organophosphorus (OP) compounds. They inhibit acetylcholinesterase (AChE), an enzyme that hydrolyses acetycholine (ACh) in the nervous system. Pathophysiological changes caused by OP poisonings are primarily the consequence of surplus ACh on cholinergic receptors and in the central nervous system. Standard treatment of OP poisoning includes combined administration of carbamates, atropine, oximes and anticonvulsants. In order to improve therapy, new compounds have been synthesised and tested. Tenocyclidine (TCP) and its adamantane derivative 1-[2-(2-thienyl)-2-adamantyl] morpholine (TAMORF) have shown interesting properties against soman poisoning. In this study, we
developed a qualitative GC-MS method to measure elimination of TCP and TAMORF through rat urine in order to learn more about the mechanisms through which TCP protects an organism from OP poisoning and to determine the duration of this protective effect. GC-MS showed that six hours after treatment with TCP, rat urine contained only its metabolite 1-thienylcyclohexene, while urine of rats treated with TAMORF contained both TAMORF and its metabolites.

Keywords

antidote; metabolite; qualitative method; urine; tenocyclidine

Hrčak ID:

49020

URI

https://hrcak.srce.hr/49020

Publication date:

17.3.2010.

Article data in other languages: croatian

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