Short communication, Note

https://doi.org/10.2478/v10007-010-0018-7

Chemical and biological investigations of Delonix regia (Bojer ex Hook.) Raf.

Israt Jahan ; Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh
Mohammad S. Rahman ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh
Mohammed Z. Rahman ; Department of Pharmacy, The University of Asia Pacific, Dhaka-1209, Bangladesh
Mohammad A. Kaisar ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh
Mohammad S. Islam ; Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh
Abdul Wahab ; Department of Skin & Veneral Diseases, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka-1000, Bangladesh
Mohammad A. Rashid ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh

Abstract

In this study five compounds, lupeol (1), epilupeol (2), β-sitosterol (3), stigmasterol (4) and p-methoxybenzaldehyde (5) were isolated from the petroleum ether and dichloromethane fractions of a methanolic extract of the stem bark of Delonix regia. Antimicrobial screening of the different extracts (15 μg mm2) was conducted by disc diffusion method. The zones of inhibition demonstrated by the petroleum ether, carbon tetrachloride and dichloromethane fractions ranged from 914 mm, 1113 mm and 920 mm, respectively, compared to kanamycin standard with the zone of inhibition of 2025 mm. In brine shrimp lethality bioassay, the carbon tetrachloride soluble materials demonstrated the highest toxicity with LC50 of 0.83 μg mL1, while petroleum ether and dichloromethane soluble partitionates of the methanolic extract revealed LC50 of 14.94 and 3.29 μg mL1, respectively, in comparison with standard vincristine sulphate with LC50 of 0.812 μg mL1. This is the first report on compounds separation from D. regia, their antimicrobial activity and cytotoxicity.

Keywords

Delonix regia (Fabaceae); methanolic extract; antimicrobial activity; brine shrimp lethality

Hrčak ID:

52252

URI

https://hrcak.srce.hr/52252

Publication date:

1.6.2010.

Article data in other languages: croatian

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