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Periodicum biologorum, Vol. 112 No. 1, 2010.

Original scientific paper

Expression of myogenic regulatory factors in rat skeletal muscles after denervation

MARINA NIKOLIĆ ; Department of Anatomy, Medical Faculty, University of Rijeka, B. Branchetta 20, 51000 Rijeka, Croatia
SNJEŽANA BAJEK ; Department of Anatomy, Medical Faculty, University of Rijeka, B. Branchetta 20, 51000 Rijeka, Croatia
DRAGICA BOBINAC ; Department of Anatomy, Medical Faculty, University of Rijeka, B. Branchetta 20, 51000 Rijeka, Croatia
TAMARA ŠOIĆ VRANIĆ ; Department of Anatomy, Medical Faculty, University of Rijeka, B. Branchetta 20, 51000 Rijeka, Croatia
GORDANA STARČEVIĆ KLASAN ; Department of Anatomy, Medical Faculty, University of Rijeka, B. Branchetta 20, 51000 Rijeka, Croatia
JURAJ ARBANAS ; Department of Anatomy, Medical Faculty, University of Rijeka, B. Branchetta 20, 51000 Rijeka, Croatia
ROMANA JERKOVIĆ ; Department of Anatomy, Medical Faculty, University of Rijeka, B. Branchetta 20, 51000 Rijeka, Croatia

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Abstract

Aim: The aim of this study was to investigate expression of myogenic
regulatory factors MyoD and myogenin during denervation in fast and slow rat skeletal muscles of different rat strains (Wistar and Sprague-Dawely).

Material and Methods: Immunohistochemical andWestern blot analyses were performed on tibialis anterior and soleus muscles.

Results: Immunohistochemical analysis of tibialis anterior and soleus
muscles during denervation demonstrated that both myonuclei and satellite cells were activated.Western blot analysis showed a significant upregulation of MyoD and myogenin proteins in m. tibialis anterior at all time points of denervation, except on 60th day for MyoD. In m. soleus during denervation Western blot analysis showed upregulation of MyoD and myogenin in first 14 days, and there was no expression after that period.

Conclusion: Our results indicate that MyoD and myogenin protein expression during denervation is muscle type specific. The role of MyoD and myogenin in adult muscle is potentially also important in orchestrating an adaptive response of existing muscle fibers during denervation.

Keywords

Hrčak ID:

52700

URI

https://hrcak.srce.hr/52700

Publication date:

31.3.2010.

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