Original scientific paper

In vitro development of buffalo preantral follicles in co-culture with cumulus or granulosa cells

Hondaravalli S. Ramesh ; National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, India
Paluru S. P. Gupta ; National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, India
Sumanta Nandi ; National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, India
Bodhaganahalli M. Manjunatha ; National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, India

Abstract

Low reproductive effifi ciency in buffalo limits the number of offspring produced during lifespan and thus results in under-exploitation of the superior female genetic material. In vitro production of transferable embryos is a method used to overcome such limitation. Developing a culture system for preantral follicles has important biotechnological implications, due to its potential for producing large number of oocytes for in vitro embryo production. Hence, the effect of the co-culture of buffalo preantral follicles with dispersed somatic cells, like cumulus cells and granulosa cells and their monolayers, was investigated in the present study. Large
preantral follicles were isolated from trypsin (1%) digested ovarian cortical slices. Cumulus and granulosa cell monolayers were prepared by culturing cells until their conflfl uency preantral follicles were co-cultured with dispersed cumulus cells (group 1), the monolayers of cumulus cells (group 2), dispersed granulosa cells (group 3) or the monolayers of granulosa cells (group 4). Large preantral follicles showed signififi cantly higher size, growth rate and survivability when co-cultured with somatic cells dispersed in the medium, as compared to those grown in a monolayer. It was concluded that dispersed somatic cells from buffalo ovarian follicles were more effective in providing support for the growth and survivability of preantral follicles in culture, compared to their monolayers in buffalo.

Keywords

preantral follicle; follicle culture; follicle development; somatic cells; monolayer; co-culture; buffalo

Hrčak ID:

53097

URI

https://hrcak.srce.hr/53097

Publication date:

8.3.2010.

Article data in other languages: croatian

Visits: 1.802 *