Original scientific paper
Evaluation of 14 Ychromosomal Short Tandem Repeat Haplotype with Focus on DYS449, DYS456, and DYS458: Czech Population Sample
Edvard Ehler
orcid.org/0000-0003-1774-0091
; Department of Anthropology and Human Genetics, Faculty of Science, Charles University in Prague, Prague, Czech Republic
Richard Marvan
; Department of Anthropology and Human Genetics, Faculty of Science, Charles University in Prague, Prague, Czech Republic
Daniel Vanek
; Forensic DNA Service, Prague, Czech Republic
Abstract
Aim To evaluate the novel triplex polymerase chain reaction
(PCR) assay for the analysis of polymorphic Y-chromosomal
short tandem repeat loci (Y-STR).
Methods A total of 14 Y-STR loci was analyzed. Allele frequencies
for 3 tetrameric Y-STR loci (DYS449, DYS456, and
DYS458) and extended haplotype loci typed by Y-PLEXTM
12 system were investigated in a sample of 50 unrelated
healthy Czech male donors. We computed the relevant intra-
population statistic parameters for our data (gene diversity,
average gene diversity over loci, and mean number
of pairwise differences) and compared our sample set with
other Central European populations using RST pairwise genetic
distance.
Results We focused on the comparison of genetic diversity
between the Y-STR extended haplotype loci and
that of the 3 additional loci, and on the benefit of using
DYS449, DYS456, and DYS458 in forensic and population
genetics applications. Total gene diversity in our sample
set was 0.998367 when using all 14 loci. Our data analysis
revealed very high genetic diversity at DYS449 locus
(0.876735), which surpasses even the diversity at DYS385a/
b (0.819592). Population comparison showed no difference
between Czech, Bavarian, Austrian, and Saxon sample
set. A minor difference was found between Czech and
Polish sample set.
Conclusion Typing of 3 Y-chromosomal microsatellite
polymorphisms may provide a useful complement to already
established sets of Y-STRs.
Keywords
Short tandem repeat; Y chromosome; Czech population; Triplex PCR; Y-STR; DYS449; DYS456; DYS458
Hrčak ID:
53507
URI
Publication date:
15.2.2010.
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