Veterinary Archives, Vol. 76 No. 5, 2006.
Original scientific paper
Detection of foot-and-mouth disease virus and identification of serotypes in East Azerbaijan province of Iran
Mehran Alamdari
Seyed Ali Ghorashi
Malahat Ahmadi
Reyhaneh Salehi-Tabar
Abstract
Three serotypes of foot-and-mouth disease virus (FMDV) including A, Asia1 and O, have been detected
in Iran. Following the mass vaccination program which was implemented in all parts of the country in 2002,
the number of outbreaks has been significantly reduced. Therefore, rapid detection of FMDV and its serotypes
in clinical samples is essential for control of new outbreaks. In this study a reverse transcription-polymerase
chain reaction (RT-PCR) was used for detection and serotyping of FMDV in clinical samples. Twelve tissue
samples were collected from suspected outbreaks from East Azerbaijan province and tested by this method.
Of 12 samples, 10 were found to be positive for FMDV. These samples were also tested in a multiplex-PCR
for serotype identification. Four samples showed to be serotype O, 4 samples identified as serotype A and 2
samples detected to be serotype Asia 1. Multiplex-PCR products were sequenced and specificity of results was
confirmed. Results indicate that in order to practice a good control measure, the RT-PCR and multiplex-PCR
could be successfully used as a robust diagnostic method.
Keywords
foot-and-mouth disease virus; polymerase chain reaction
Hrčak ID:
5150
URI
Publication date:
20.10.2006.
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