Original scientific paper

Laser Induced Fluorescence of Carious Lesion Porphyrins

Danijela Matošević orcid.org/0000-0003-3104-5749 ; School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Zrinka Tarle ; School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Snežana Miljanić ; Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb, Croatia
Zlatko Meić ; Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb, Croatia
Lana Pichler ; Laboratory for Femtosecond Laser Spectroscopy, Institute of Physics, Zagreb, Croatia
Goran Pichler ; Laboratory for Femtosecond Laser Spectroscopy, Institute of Physics, Zagreb, Croatia

Abstract

Objectives: This paper reports the preliminary results of the measurements of laser induced fluorescence in several porphyrin solutions. Coproporphyrin and uroporphyrin are common constituents
of carious lesions. Their property to exhibit fluorescence when irradiated with a light of certain wavelength could be used as a means to detect carious lesions. Materials and methods: Absorption coefficient measurements of coproporphyrin I dihydrochloride and uroporphyrin I dihydrochloride solutions were performed under different pH conditions in order to identify spectral regions for effective laser excitation. Lasers with discrete wavelengths at 420 nm, 473 nm and 532 nm were used for the induction of the fluorescence. Results: At all laser wavelengths interesting fluorescence bands peaking at 591 nm, 619 nm and 652 nm for coproporphyrin and at 617 nm and 680 nm for uroporphyrin were observed. Conclusions: When combined together all bands should correspond to the spectral band structures found in real carious lesions.

Keywords

Porphyrins; Coproporphyrins; Uroporphyrins; Dental Caries; Spectrometry, Fluorescence

Hrčak ID:

55633

URI

https://hrcak.srce.hr/55633

Publication date:

18.6.2010.

Article data in other languages: croatian

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