Original scientific paper

Evaluation of CD45+ cells kinetics in the blood of fattening chickens immunized with live or inactivated Newcastle disease vaccine

Maja Popović ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Mirta Balenović ; Poultry Centre, Croatian Veterinary Institute, Zagreb, Croatia
Anamaria Ekert Kabalin ; Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Vladimir Savić ; Poultry Centre, Croatian Veterinary Institute, Zagreb, Croatia
Nada Vijtiuk ; Teacher Education Faculty, University of Zagreb, Zagreb, Croatia
Ksenija Vlahović ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivica Valpotić ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

Since insight into the effects of Newcastle disease vaccination on cellular immune responses has been insufficient to date, this study follows the leukocyte dynamics in the blood of fattening chickens inoculated against Newcastle disease with lyophilised live vaccine of the La Sota virus strain (experimental group E1) and/or inactivated vaccine produced from the same strain (experimental group E2). Blood was treated with labelled antibody for the CD45+ leukocyte marker and examined by flfl ow citometry. In bouth experimental a statistically
signififi cant increase (P<0.01) of CD45+ cells was observed in relation to the control group of chickens, and the increase was more intensive and faster in the chickens vaccinated with lyophilised live vaccine than it was in chickens vaccinated with inactivated vaccine. The group treated with live vaccine had a signififi cantly increased number of CD45+ cells 6, 12, and 24 hr, and 7, 14, 21 and 28 days after inoculation. But on the 14th day after vaccination, a signififi cantly increased number of CD45+ cells (P<0.05) was found in the chickens inoculated with inactivated vaccine. It can be concluded that the lyophilised live vaccine was more efficient and cellular immunity was achieved sooner than with the inactivated vaccine during the observed period of 28 days after inoculation.

Keywords

cellular immunity; flow cytometry; fattening chickens; Newcastle disease; vaccines

Hrčak ID:

55677

URI

https://hrcak.srce.hr/55677

Publication date:

8.3.2010.

Article data in other languages: croatian

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