Original scientific paper

Trace Detection of Diphenhydramine by Adsorption on a Microelectrode at Flow Injection System by Fast Fourier Transform Continuous Cyclic Voltammetry

Parviz Norouzi ; Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran; Endocrinology & Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Reza Ganjali ; Endocrinology & Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran; Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
Ezzattollah Nouryousefi ; Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
Seyed Jamaleddin Shahtaheri ; Department of Public Health, Center for Envir. Research, School of Public Health, Tehran Univ. of Medical Sciences, Tehran, Iran
Rassoul Dinarvand ; Medicinal Nanotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

Abstract

A continuous cyclic voltammetric study of diphenhydramine at gold micro electrode was carried
out. Some investigations were also done to find the effects of various parameters on the sensitivity of the
proposed method. The experiments were performed under the following conditions: pH = 2, the scan rate
= 40 V/ s (v), accumulation potential = 500 mV (E), and accumulation time = 0.2 s (t). The drug in phosphate
buffer (pH = 2.0) is adsorbed at optimized condition on the surface of electrode, giving rise to
change in the current of well-defined oxidation peak of gold in the flow injection system. The proposed
detection method is a very fast and appropriate technique for determination of the drug compound in a
wide variety of chromatographic analysis methods. Signal-to-noise ratio has significantly increased by application of discrete Fast Fourier Transform (FFT) method, background subtraction and twodimensional
integration of the electrode response over a selected potential range and time window. The linear concentration range was from 4.0 × 10–7 to 1.0 × 10–11 mol dm–3 (r = 0.9987) with a limit of
detection and quantitation 5 × 10–12 and 4 × 10–11 mol dm–3, respectively. The method has the requisite
accuracy, sensitivity, precision and selectivity to assay diphenhydramine in tablets.

Keywords

diphenhydramine; countinuous cyclic voltammetry; flow injection; fourier transformation

Hrčak ID:

56016

URI

https://hrcak.srce.hr/56016

Publication date:

15.7.2010.

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