Original scientific paper

CYP1A expression in the hepatopancreas of Mullus barbatus, Merluccius merluccius and Trigla lucerna at the mouth of the river Bojana

MIRJANA MIHAILOVIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
NEVENA GRDOVIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
SVETLANA DINIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
ALEKSANDRA USKOKOVIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
MELITA VIDAKOVIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
ILIJANA GRIGOROV ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
DESANKA BOGOJEVIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
SVETLANA IVANOVIĆ-MATIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
Miodrag PETROVIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
SVETLANA LABUS-BLAGOJEVIĆ ; Institute for Public Health, »Milan Jovanović-Batut«, Doktora Subotića 5, 11000 Belgrade, Serbia
VESNA MARTINOVIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
JELENA ARAMBAŠIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia
GORAN POZNANOVIĆ ; Molecular Biology Laboratory, Institute for Biological Research »Siniša Stanković«, University of Belgrade, Despot Stephen Blvd. 142, 11060 Belgrade, Serbia

Abstract

Background and Purpose: CYP1A is one of the most sensitive biomarkers in fish of exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). These compounds induce a dose-dependent transcriptional induction of the CYP1A gene that results in increased protein concentrations. The aim of this study was the detection of changes in CYP1A protein levels as a result of the exposure of fish to PAHs and PCBs at the mouth of the river Bojana in the spring.

Materials and Methods: The PAH and PCB contents in seawater and
sediment were determined by gas chromatography. CYP1A induction was examined in the hepatopancreas of Red mullet (Mullus barbatus), European hake (Merluccius merluccius) and Tub gurnard (Trigla lucerna) by immunoblot analysis.

Results and Conclusions: Chemical analyses of seawater and sediment revealed the presence of several PAHs and PCBs. CYP1A was detected in the hepatic microsomal fraction in all examined fish species as an adaptive response to the presence of pollutants. The reported changes at the molecular level represent an early-warning signal that reflected the biological response of fish to elevated toxin concentrations in the environment.

Keywords

CYP1A; fish; hepatopancreas; PAH, PCB

Hrčak ID:

58143

URI

https://hrcak.srce.hr/58143

Publication date:

1.6.2010.

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