Original scientific paper

Effects of different cytokinins on chlorophyll retention in the moss Bryum argenteum (Bryaceae)

ANETA SABOVLJEVIĆ ; Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
MARKO SABOVLJEVIĆ ; Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
VANJA VUKOJEVIĆ ; Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia

Abstract

Background and Purpose: Cytokinins are a group of plant hormones
that have an important role in plant growth and developmental processes.
Chlorophyll content is an extremely important parameter in estimating the plant production level. Since bryophytes do not have such economical importance as vascular plants and their production in many ecosystems is small, they remain uninteresting for studying their chlorophyll level. The aim of this study was to compare the effect of different cytokinins on chlorophyll retention in moss B. argeteum gametophyte shoots grown in natural conditions with those grown in in vitro culture.

Material and Methods: The effect of different cytokinins: kinetin
(KIN), 6-benzylaminopurine (BAP) and thidiazuron (TDZ) on chlorophyll retention of the moss Bryum argenteum Hedw. (Bryaceae) derived from in vitro culture or grown in nature was tested. Plants derived from in vitro culture were grown on Murashige and Skoog (MS) medium at 25±2°C. Gametophyte shoots were used in experiments where influence of different concentrations (0.001–10 μM) of three cytokinins was used to investigate their effect on chlorophyll-a, -b and total chlorophyll retention.

Results and Conclusions: Cytokinins had a positive but unequal influence on chlorophyll retention in both plant groups – plants derived from in vitro culture and plants grown in the nature. Kinetin proved to be the most effective cytokinin in chlorophyll retention. Exogenous application of kinetin increased chlorophyll content with concentration (0–10μM). BAP had similar trends in in vitro and native mosses, increasing chlorophyll content up to 1 μM and then significantly decreasing, although the chlorophyll content was greater in in vitro grown plants. TDZ showed significantly better effect in in vitro cultured moss shoots, but when applied in concentrations
higher than 0.1 μM, total chlorophyll content decreased.

Keywords

Bryum argenteum; chlorophyll; cytokinins; moss; retention

Hrčak ID:

58275

URI

https://hrcak.srce.hr/58275

Publication date:

1.9.2010.

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