Original scientific paper

A high performance liquid chromatography (HPLC) method with evaporative light scattering detector for quantififi cation of major phospholipids classes of donkey serum

Hisham I. Seri ; Department of Clinical Studies, Faculty of Veterinary Medicine, University of Sudan of Science and Technology (SUST), Sudan
Hassan Y. Naim ; Institut für Physiologische Chemie, Tierärztliche Hochschule Hannover, Hannover, Germany
Tigani Hassan ; Department of Medicine Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Khartoum, Sudan

Abstract

An HPLC method with an evaporative laser scattering detector was used to quantify major phospholipids fractions in donkeys’ serum. Blood samples were collected bimonthly for a whole year from 20 donkeys (10 male and 10 female) from the Sudanese breed kept at the premises of the Central Veterinary Research Laboratory (CVRL), Soba. The method used made the excellent separation possible of phosphatidylglycerine (PG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and sphingomyelin (SM). in 27 minutes, including the regeneration of the column. The SM resulted in two peaks (saturated and unsaturated fatty acids), as described by other researchers. The method was unable to separate phosphatidylserine (PS), which appeared in one peak with phosphatidylinositol. There is a signififi cant difference in the level of PG, PE, PI and SM between females and males.The method fully discussed in this study and the obtained values of major phospholipids can be used for health control and diagnosis of diseases.

Keywords

donkeys; ELSD; HPLC; phospholipid; sphingolipid; Sudan

Hrčak ID:

58452

URI

https://hrcak.srce.hr/58452

Publication date:

16.6.2010.

Article data in other languages: croatian

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