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Veterinary Archives, Vol. 80 No. 4, 2010.

Original scientific paper

In vitro cultivation of porcine limbal transplants

Boris Pirkić orcid id orcid.org/0000-0002-5137-9028 ; Clinic for Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Ksenija Vlahović ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Marko Hohšteter ; Department of Pathology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Mirna Tominac ; University Clinic for Traumatology, Medical Faculty, University of Zagreb, Croatia
Ante Muljačić ; University Clinic for Traumatology, Medical Faculty, University of Zagreb, Croatia
Daniel Špoljarić ; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Njetočka Gredelj Šimec ; General Hospital, Karlovac, Croatia
Mario Kreszinger ; Clinic for Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Marko Stejskal ; Clinic for Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Croatia
Maja Popović

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Abstract

The stem cells that provide tissue regeneration are residents of different organ structures in the body and are usually located in well-protected sites of organs and tissues. For stem cells of either corneal epithelium such sites are in the basal epithelial layer of the cornea residing in its limbal region, termed the palisade of Voight. The growing interest in allografts and xenografts implies a thorough study of the regenerative potential of these cells, as well as a clear description of their patterns in the in vitro tissue cultures to be grafted. The aim of this study was to evaluate the culturing patterns of porcine limbal stem cells and to optimize growing conditions of these cell cultures, in order to develop a reliable biomedical model intended for studying the potentials of allografts/xenografts originated from porcine tissues. Porcine stem cell equivalents have potential in reparative/regenerative veterinary medicine for pets, horses and selected breeds of food animals, but also as xenografts for humans.

Keywords

porcine; cornea; stem cells

Hrčak ID:

58460

URI

https://hrcak.srce.hr/58460

Publication date:

28.7.2010.

Article data in other languages: croatian

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