Professional paper

Activity of Radiofrequency Radiation at the Cell Level

Ivančica Trošić ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Ivan Pavičić ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Ivana Bušljeta ; Belupo d.d., Zagreb, Hrvatska
Mirjana Mataušić-Pišl ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Sanja Milković-Kraus ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska

Abstract

The aim was to assess the biological effect of low-intensity, radiofrequency (RF) radiation on cell macromolecular structures, DNA, and cytoskeleton fi bres, including the microtubule, actin, and vimentin. The study was performed both in vivo (animal model) and in vitro (cell culture). The in vivo design included exposed rats (n=40) and unexposed controls (n=24). Both groups were divided in four subgroups. The fi rst exposed subgroup was irradiated with 2450 MHz RF waves and an average power density of 7.5 mW cm-2 (SAR = 1.25 W kg-1) for 4 h in total, the second for 16 h, the third for 30 h, and the fourth for 60 h. Daily exposure in all groups was 2 h a day. The animals were killed on exposure days 2, 8, 15, and 30. The in vitro design included continuous cell culture of V79 cells exposed to a 915 MHz RF fi eld and 10 V m-1 electric fi eld (SAR = 0.2 W kg-1) for 1 h, 2 h, and 3 h. We performed the micronucleus test on polychromatic erythrocytes in vivo and V79 cells in vitro. The test showed signifi cantly more polychromatic erythrocytes with micronuclei (MN) in rats irradiated for 16 h.
The in vitro study confi rmed the signifi cantly higher occurrence of micronucleated cells as well as damage to polar microtubules and actin fi laments, which correlated with the duration of radiation exposure. Our in vivo and in vitro results confirm the possibility of undesired effects of man-made radiofrequency fields at the cell level.

Keywords

low-intensity irradiation; micronucleus test; RF band

Hrčak ID:

60289

URI

https://hrcak.srce.hr/60289

Publication date:

9.11.2010.

Article data in other languages: croatian

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