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Archives of Industrial Hygiene and Toxicology, Vol. 61 No. Supplement, 2010.

Professional paper

DNA Repair Gene Polymorphisms and Sensitivity to Ionising Radiation

Mirta Milić orcid id orcid.org/0000-0002-9837-7185 ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Ružica Rozgaj ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Vilena Kašuba ; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Dragan Kubelka ; Državni zavod za radiološku i nuklearnu sigurnost, Zagreb, Hrvatska
Sabrina Angelini ; Department of Pharmacology, University of Bologna, Italija
Patrizia Hrelia ; Department of Pharmacology, University of Bologna, Italija

Full text: croatian pdf 138 Kb

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Abstract

Increasing exposure to ionising radiation raises a great concern about potential DNA damage in occupationally exposed individuals. Polymorphisms of DNA repair genes can determine individual
sensitivity and DNA damage response to low doses of ionising radiation. The objective of this study was to assess DNA damage in leukocytes at baseline, immediately after and 120 min after exposure to gamma-radiation of 2 Gy, to compare DNA damage between the control group of subjects and subjects occupationally exposed to low-dose gamma-radiation, and to determine the relationship between hOGG1 (8-oxoG specifi c DNA glycosylase/AP-Lyase, Ser326Cys), XRCC1 (X-ray repair cross-complementing protein-group 1, Arg194Trp), and MGMT(O6-methylguanine-DNA methyltransferase, Leu84Phe) gene and DNA damage. The study enrolled 40 healthy subjects (20 controls and 20 occupationally exposed subjects), whose leukocytes were exposed to ionising radiation and tested for DNA damage (tail length, percentage od DNA in comet tail, and tail moment) using the alkaline version of the comet assay. Our results
show that tail DNA percentage and tail moment were signifi cantly higher in the exposed group at baseline, immediately after, and 120 min after exposure to 2 Gy. The exposed subjects carrying polymorphic alleles had signifi cantly higher DNA damage than homozygous carriers of the same gene and controls. Combined use of the alkaline comet assay and genotyping of DNA repair genes could help discover sensitive occupationally exposed individuals who can accumulate higher DNA damage and are at higher risk of developing tumours.

Keywords

comet assay; DNA damage; hOGG1; genotyping; XRCC1; MGMT

Hrčak ID:

60303

URI

https://hrcak.srce.hr/60303

Publication date:

9.11.2010.

Article data in other languages: croatian

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