Original scientific paper
Equine Butyrylcholinesterase Protects Rats against Inhalation Exposure to Sublethal Sarin Concentrations
Jři Bajgar
Lucie Bartošova
Josef Fusek
Daniel Jun
Kamil Kuča
Abstract
Protection experiments were conducted using different doses of equine serum butyrylcholinesterase (Eq
BuChE) as pretreatment in rats. Cholinesterase activities were determined in blood [whole blood, red blood
cells (RBC) acetylcholinesterase (AChE), and plasma BuChE] before and after sarin inhalation exposure
in untreated rats and those pretreated with Eq BuChE. Brain AChE activity was also determined in the
frontal cortex, basal ganglia and pontomedullar areas following exposure. Dose-dependent increases in
plasma BuChE activity and no changes in the RBC and brain AChE activities were demonstrated following
i.p. injection of different amounts of Eq BuChE. Decreases in plasma BuChE activity and RBC and brain
AChE activities were observed in control rats following sarin inhalation exposure. In rats pretreated with
Eq BuChE this inhibition was lower than in control animals. These results demonstrate protective effects
of Eq BuChE pretreatment in rats intoxicated with sublethal concentrations of sarin by inhalation.
Keywords
butyrylcholinesterase; rats; inhalation exposure; sarin; acetylcholinesterase; basal ganglia; frontal cortex; plasma; pontomedullar area; red blood cells; whole blood
Hrčak ID:
5976
URI
Publication date:
13.12.2006.
Visits: 1.685 *