Original scientific paper
https://doi.org/10.2478/10004-1254-61-2010-2058
Determination of Benzodiazepines in Urine Via Benzophenone Derivatives Using Liquid Chromatography-Tandem Mass Spectrometry
Robert Roškar
; Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Marija Sollner Dolenc
; Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Abstract
The aim of this study was to validate a new method for determining benzodiazepines in urine via their benzophenone derivatives, based on liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Selected benzodiazepines were analysed after acid hydrolysis of urine and extraction by ethyl acetate in the presence of an internal standard. Samples were analysed using electrospray ionization LC-MS/MS in a multiple reaction monitoring mode. The chromatographic run time on a reversed phase C18 analytical column was set for 9 min. This method was validated in 21 patients receiving methadone. Benzodiazepines intake was established in two out of three patients. LC-MS/MS results were also compared with the rapid immunoassay and the methods showed good agreement. However, in three cases benzodiazepines were detected by LC-MS/MS, but not by the immunoassay. The sensitivity of the developed LC-MS/MS method is comparable to or even higher than of previously reported methods, which makes it suitable as a confi rmatory method.
Keywords
benzodiazepine metabolites; diazepam; drug abuse; lorazepam; methadone treatment
Hrčak ID:
62451
URI
Publication date:
21.12.2010.
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