Preliminary communication

Chemical Differentiation of Berberis croatica and B. vulgaris Using HPLC Fingerprinting

Marijana Zovko Končić ; Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Dario Kremer ; Department of Pharmaceutical Botany with Pharmaceutical Botanical Garden ''Fran Kušan'', Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Wolfgang Schühly ; Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 4, 8010 Graz, Austria
Adelheid Brantner ; Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 4, 8010 Graz, Austria
Ksenija Karlović ; Department for Ornamental Plants and Landscape Architecture, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Zdenka Kalođera ; Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia

Abstract

In Croatia, two indigenous species of the genus Berberis L. occur: common barberry (B. vulgaris
L.) and Croatian barberry (B. croatica Horvat), endemic to the Balkano-Illyric region. As a contribution
to the chemotaxonomy of the two species, differences of their RP-HPLC chromatograms were investigated.
A HPLC method combined with Unweighted Pair Group Method with Arithmetic means (UPGMA)
was developed for species differentiation and compared to several previously described procedures
for the differentiation of other species. The results indicate that common and Croatian barberry are closely
related species, but can be differentiated according to their HPLC fingerprints under appropriate chromatographic
conditions and selection of clustering variables. Generally, the amount of berberine was higher
in samples obtained from B. vulgaris than from B. croatica.

Keywords

Berberis croatica; Berberis vulgaris; HPLC; chemotaxonomy

Hrčak ID:

62651

URI

https://hrcak.srce.hr/62651

Publication date:

1.12.2010.

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