Acta Pharmaceutica, Vol. 61 No. 1, 2011.
Original scientific paper
https://doi.org/10.2478/v10007-011-0004-8
Reduction in cisplatin genotoxicity (micronucleus formation) on non target cells in mice by protransfersome gel formulation used for management of cutaneous squamous cell carcinoma
VANDANA GUPTA
; School of Pharmaceutical Sciences, Rajiv Gandhi Technical University, Airport Bypass Road, Gandhi Nagar, Bhopal (MP)-462036, India
RAMESH CHANDRA Agrawal
; Jawaharlal Nehru Cancer Hospital and Research Center, Post Box. No. 32, Idgah Hills, Bhopal-462001 (MP) India
PIYUSH TRIVEDI
; School of Pharmaceutical Sciences, Rajiv Gandhi Technical University, Airport Bypass Road, Gandhi Nagar, Bhopal (MP)-462036, India
Abstract
Cisplatin-loaded protransfersome system was prepared and characterized for in vitro drug permeation, drug deposition and antitumor effect. A histopathological study and a genotoxicity study were also done. The skin permeation data from protransfersome gel formulation of cisplatin revealed 494.33 ± 11.87 µg cm-2 drug permeation, which was significantly higher than that from the control plain drug solution in 0.9 % NaCl (p < 0.001). Untreated group of animals showed invasive moderately differentiated keratinizing squamous cell carcinoma (malignant stage). However, with cisplatin loaded protransfersome gel system simple epithelial hyperplasia (pre-cancerous stage) with no cancerous growth was observed. Also, a significant induction in micronucleus formation was found in the group that was treated with injectable intraperitoneal cisplatin preparation in 0.9 % saline as compared to the group treated with topical protransfersome gel formulation. The findings of this research work appears to support the improved, site-specific and localized drug action in the skin, thus providing a better option to deal with skin related problems like squamous cell carcinoma.
Keywords
cisplatin; protransfersome; topical chemotherapy; genetic damage; bone marrow cells; micronucleated polychromatic erythrocytes
Hrčak ID:
63373
URI
Publication date:
1.3.2011.
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