Medica Jadertina, Vol. 40 No. 3-4, 2010.
Original scientific paper
Determination of ochratoxin A in barley-Contribution to the mycotoxin hypothesis on the etiology of endemic nephropaty
Iva Pavlinić
; Institut za zaštitu bilja, Zagreb
Dinko Puntarić
; Zavod za javno zdravstvo "Dr. A.Štampar",Zagreb,Služba za zdravstvenu ekologiju
Jasna Bošnir
; Zavod za javno zdravstvo "Dr.Andrija Štampar",Zagreb,Služba za zdravstvenu ekologiju
Dario Lasić
; Zavod za javno zdravstvo,Zagreb,Služba za zdravstvenu ekologiju
Lidija Barušić
; Zavod za javno zdravstvo "Dr.Andrija Štampar",Zagreb,Služba za zdravstvenu ekologiju
Matijana Jergović
; Zavod za javno zdravstvo "Dr.Andrija Štampar", Zagreb,Služba za zdravstvenu ekologiju
Maja Miškulin
; Sveučilište J.J.Strossmayera u Osijeku, Medicinski fakultet Osijek
Ida Puntarić
; Medicinski fakultet Zagreb
Katarina Vučić
; Hrvatski zavod za zdravstveno osiguranje Zagreb
Abstract
The aim of the study was to determine ochratoxin A (OTA) concentration in barley from the Slavonski Brod area endemic for the Balkan endemic nephropathy (BEN) in comparison with barley from Osijek and Nova Gradiška areas free from BEN. Thin-layered chromatography and high-performance liquid chromatography were used on OTA detection in barley samples collected in 2000 and 2002 at Slavonski Brod, Osijek and Nova Gradiška locations. Considering the maximum allowable concentration (MAC) of 5 μg/kg for cereals, the highest OTA concentrations were determined in barley samples collected in the Slavonski Brod area in 2000 (median 25 μg/kg; confidence interval 20.0 – 25.0 μg/kg), followed by barley samples collected in the same area in 2002 (median 20 μg/kg; confidence interval 10.0 – 20.0 μg/kg) (p < 0.001). In the Osijek area, the median OTA concentration in barley samples from 2000 was 12.5 μg/kg (confidence interval 10.0 – 20.0 μg/kg), whereas barley samples collected in 2002 were free from OTA as were those collected in both 2000 and 2002 in the Nova Gradiška area. Comparison of barley samples in separate Slavonski Brod and Osijek areas yielded statistically significant differences in OTA concentration
between the samples collected in 2000 and 2002. Mutual comparison of samples according to the sampling locations established a statistically significant difference between the samples collected in the Slavonski Brod area and those from non-endemic areas in 2000 and 2002. Grain humidity did not exceed the allowable level of 14% in any of study samples. In the Slavonski Brod area, barley contamination with OTA exceeded MAC several fold and was significantly higher in the Slavonski Brod area than in other study areas which suggests the possible connection between BEN and OTA contamination of cereals and mycotoxins in general.
Keywords
mycotoxins; ochratoxin A; endemic nephropaty; cereals; barley, Croatia
Hrčak ID:
65088
URI
Publication date:
1.3.2011.
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