Original scientific paper
https://doi.org/10.2478/10004-1254-62-2011-2082
Antioxidative Effects of N-acetylcysteine, Lipoic Acid, Taurine, and Curcumin in the Muscle of Cyprinus carpio L. Exposed to Cadmium
Yusuf Sevgiler
; Adiyaman University, Faculty of Science and Letters, Department of Biology, Adiyaman, Turkey
Sahire Karaytug
; University of Mersin, Faculty of Aquaculture, Yenişehir Campus, Mersin, Turkey
Fahri Karayakar
; University of Mersin, Faculty of Aquaculture, Yenişehir Campus, Mersin2, Turkey
Abstract
We investigated the muscle tissue of a teleost Cyprinus carpio L. to find out whether N-acetylcysteine (NAC), alpha-lipoic acid (LA), taurine (TAU), and curcumin (CUR) were able to counteract oxidative
stress induced by acute exposure to cadmium (Cd). The muscle tissue was dissected 96 h after a single intraperitoneal injection of Cd (5 mg kg-1) and of antioxidant substances (50 mg kg-1). Using
spectrophotometry, we determined the glutathione redox status, lipid peroxidation levels and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione disulphide reductase (GR). Accumulation of Cd in the muscle was analysed using inductively coupled plasma - optical emission spectrometry (ICP-OES).
All substances lowered Cd levels in the following order of effi ciency; LA=NAC>TAU=CUR. Cadmium increased SOD activity, but CAT activity declined, regardless of antioxidant treatment. Treatment with
CUR induced GPx activity. Treatment with TAU lowered Cd due to higher total glutathione (tGSH). The most effective substances on lipid peroxidation were LA and NAC due to a greater Cd-lowering potential.
It seems that the protective role of TAU, LA, and NAC is not necessarily associated with antioxidant enzymes, but rather with their own activity.
Keywords
accumulation; antioxidants; fish; muscle; oxidative stress
Hrčak ID:
65308
URI
Publication date:
16.3.2011.
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