Review article
https://doi.org/10.2478/10004-1254-62-2011-2052
Chemical Methods for Determination of Hydroxylated Metabolites of Polycyclic Aromatic Hydrocarbons and Polychlorinated Biphenyls in Biological Material
Darija Klinčić
; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Snježana Herceg Romanić
; Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska
Abstract
This review presents methods for the analysis of hydroxylated metabolites of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in humans and animals. These metabolites serve as biomarkers of human and animal exposure to the mentioned pollutants, but some metabolites also have toxic properties.
Most are analysed in urine, which is the most accessible sample, but they can also be analysed in human and animal liver, bile, and adipose tissue. Their analysis is important for assessing bioavailability of aromatic pollutants and their toxicity in human
organism, but also the toxicity of metabolites themselves. Advancements in analytical methods have made it possible to analyse multiple metabolites in a sample at the same time. New extraction techniques and more precise and selective qualitative and quantitative analyses can now detect very low metabolite oncentrations. An extra advantage is that these simple techniques require less chemicals and time.
Keywords
analytical procedures; biomarkers; metabolism; PAH; PCB; pollutants; toxicity
Hrčak ID:
65317
URI
Publication date:
16.3.2011.
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