Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-64-2013-2365
Anticancer and Antioxidant Properties of Terpinolene in Rat Brain Cells
Elanur Aydin
; Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey
Hasan Türkez
; Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey
Şener Taşdemir
; Department of Medical Genetics, Ataturk University, Erzurum, Turkey
Sažetak
Terpinolene (TPO) is a natural monoterpene present in essential oils of many aromatic plant species. Although various biological activities of TPO have been demonstrated, its neurotoxicity has never been explored. In this in vitro study we investigated TPO’s antiproliferative and/or cytotoxic properties using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) test, genotoxic damage potential using the single-cell gel electrophoresis (SCGE), and oxidative effects through total antioxidant capacity (TAC) and total oxidative stress (TOS) in cultured primary rat neurons and N2a neuroblastoma cells. Dose-dependent effects of TPO (at 10 mg L-1, 25 mg L-1, 50 mg L-1, 100 mg L-1, 200 mg L-1, and 400 mg L-1) were tested in both cell types. Significant (P<0.05) decrease in cell proliferation were observed in cultured primary rat neurons starting with the dose of 100 mg L-1 and in N2a neuroblastoma cells starting with 50 mg L-1. TPO was not genotoxic in either cell type. In addition, TPO treatment at 10 mg L-1, 25 mg L-1, and 50 mg L-1 increased TAC in primary rat neurons, but not in N2a cells. However, at concentrations above 50 mg L-1 it increased TOS in both cell types. Our findings clearly demonstrate that TPO is a potent antiproliferative agent for brain tumour cells and may have potential as an anticancer agent, which needs to be further studied.
Ključne riječi
antiproliferative agent; comet assay; MTT assay; neurotoxicity; N2a neuroblastoma cell line; oxidative status
Hrčak ID:
107633
URI
Datum izdavanja:
20.9.2013.
Posjeta: 2.966 *