Ostalo

https://doi.org/10.3325/cmj.2017.58.203

Results of a collaborative study on DNA identification of aged bone samples

Daniel Vanek ; Forensic DNA Service, Prague, Czech Republic
Bruce Budowle ; Center for Human Identification, University of North Texas Health Science Center, TX 76107, USA
Jitka Dubska-Votrubova ; Forensic DNA Service, Prague, Czech Republic
Angie Ambers ; Center for Human Identification, University of North Texas Health Science Center, TX 76107, USA
Jan Frolik ; Institute of Archaeology AS CR, Prague
Martin Pospisek ; Biologicals, Ricany, Czech Republic
Ahmed Anwar Al Afeefi ; Forensic Evidence Department, Abu Dhabi Police General Directorate, Ministry of Interior, United Arab Emirates
Khalid Ismaeil Al Hosani ; Forensic Evidence Department, Abu Dhabi Police General Directorate, Ministry of Interior, United Arab Emirates
Marie Allen ; Dept. of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala university, Biomedical centre, Uppsala, Sweden
Khudooma Saeed Al Naimi ; Forensic Evidence Department, Abu Dhabi Police General Directorate, Ministry of Interior, United Arab Emirates
Dina Al Salafi ; Sharjah Forensic Laboratory, Sharjah Police H.Q, Ministry of Interior, United Arab Emirates
Wafa Ali Rashid Al Tayyari ; Forensic Evidence Department, Abu Dhabi Police General Directorate, Ministry of Interior, United Arab Emirates
Wendy Arguetaa ; Forensic Genetics Department, Fundaci
Michel Bottinelli ; Laboratorio di Diagnostica Molecolare, Gentilino, Switzerland
Magdalena M. Bus ; Dept. of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala university, Biomedical centre, Uppsala, Sweden
Jan Cemper-Kiesslich
Olivier Cepil
Greet De Cock ; Nationaal Instituut voor Criminalistiek en Criminologie, Brussels, Belgium
Stijn Desmyter ; Nationaal Instituut voor Criminalistiek en Criminologie, Brussels, Belgium
Hamid El Amri ; Genetic Laboratory of Royal Gendarmerie, Rabat, Morocco
Hicham El Ossmani ; Genetic Laboratory of Royal Gendarmerie, Rabat, Morocco
Ruth Galdies ; Laboratory of Molecular Genetics and the Malta BioBank, Department of Phsyiology & Biochemistry, Biomedical Sciences Building, University of Malta, Msida, Malta
Sebastian Grn ; Bayerisches Landeskriminalamt, M
Francois Guidet ; Forensic DNA Service, Prague, Czech Republic
Anna Hoefges ; Laboratory of Molecular Genetics and the Malta BioBank, Department of Phsyiology & Biochemistry, Biomedical Sciences Building, University of Malta, Msida, Malta
Cristian Bogdan Iancu ; National Institute of Legal Medicine
Petra Lotz ; Bayerisches Landeskriminalamt, M
Alessandro Maresca ; Laboratorio di Diagnostica Molecolare, Gentilino, Switzerland
Marion Nagy ; Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charite, Universit
Jindrich Novotny ; Bayerisches Landeskriminalamt, M
Hajar Rachid ; Genetic Laboratory of Royal Gendarmerie, Rabat, Morocco
Jessica Rothe ; Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charite, Universit
Marguerethe Stenersen ; Division of Forensic Sciences/Forensic Genetics, Norwegian Institute of Public Health, Oslo, Norway
Mishel Stephenson ; Forensic Genetics Department, Fundaci
Alain Stevanovitch ; Institut National de Police Scientifique, Laboratoire de Police Scientifique de Marseille, Marseille, France
Juliane Strien ; Institute of Legal Medicine, Jena University Hospital-Friedrich Schiller University Jena, Jena, Germany
Denilce R. Sumita ; Genomic Engenharia Molecular, S
Joanna Vella ; Laboratory of Molecular Genetics and the Malta BioBank, Department of Phsyiology & Biochemistry, Biomedical Sciences Building, University of Malta, Msida, Malta
Judith Zander ; Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charite, Universit

Sažetak

AimA collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. MethodsEighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. ResultsAlthough the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. ConclusionThe laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.

Ključne riječi

Hrčak ID:

183990

URI

https://hrcak.srce.hr/183990

Datum izdavanja:

7.7.2017.

Posjeta: 1.675 *