Acta clinica Croatica, Vol. 58. No. 4., 2019.
Izvorni znanstveni članak
https://doi.org/10.20471/acc.2019.58.04.11
Effect of Sun Exposure Duration on Cyclooxygenase-2 Expression in the Conjunctiva
Patricia Reisz Majić
orcid.org/0000-0002-7795-1790
; Department of Ophthalmology and Optometry, Faculty of Medicine, Osijek University Hospital Centre, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Branimir Cerovski
; Department of Ophthalmology and Optometry, School of Medicine, Zagreb University Hospital Centre, Zagreb, Croatia
Petar Majić
; Department of Radiology, Vukovar General Hospital, Vukovar, Croatia
Ivan Krković
; Department of Ophthalmology and Optometry, Faculty of Medicine, Osijek University Hospital Centre, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Josip Barać
; Department of Ophthalmology and Optometry, Faculty of Medicine, Osijek University Hospital Centre, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Ivanka Bradvica Štenc
; Department of Neurology, Faculty of Medicine, Osijek University Hospital Centre, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Sažetak
Pterygium is a multifactorial proliferative pathologic change of bulbar conjunctiva. The purpose of this study was to determine the effect of the duration of exposure to solar radiation (expressed in years) on the incidence of positive cyclooxygenase-2 (COX-2) finding in conjunctival
tissue in two groups of patients. Group 1 consisted of 68 patients with primary pterygium having undergone conjunctival autograft transplantation. Group 2 consisted of 43 patients with morphologically
unchanged conjunctivae that were harvested during cataract surgery. All 111 specimens were submitted to immunohistochemical staining for monoclonal COX-2 antibody (1:100; M361701 MO A-HU, Dako, Santa Clara, California, USA). The immunostaining intensity in the surface epithelium was scored using the following grading system: 0 no immunostaining; + weak immunostaining (few cells being positive focally or scattered); ++ medium immunostaining; and +++ strong immunostaining (diffuse staining throughout the tissue). The analysis of COX-2 activity yielded 29 (42.6%) positive findings in group 1 and 27 (62.8%) positive findings in group 2. Group 2 consisted of statistically
significantly older individuals with a history of considerably longer sun exposure. Statistical analysis proved the duration of exposure to solar radiation to be the most important factor in positive COX-2 findings.
Ključne riječi
Conjunctiva; Cyclooxygenase 2; Pterygium; Sunlight – adverse effects
Hrčak ID:
235155
URI
Datum izdavanja:
1.12.2019.
Posjeta: 2.157 *