Izvorni znanstveni članak

https://doi.org/10.11613/BM.2020.030704

Test results comparison and sample stability study: is the BD Barricor tube a suitable replacement for the BD RST tube?

Sanja Mandić orcid.org/0000-0003-3921-8181 ; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia; Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia
Dario Mandić ; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia; Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia
Iva Lukić ; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia; Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia
Tara Rolić ; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia; Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia
Vesna Horvat ; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia; Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia
Maja Lukić ; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia; Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia
Silvija Osvald ; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia; Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia
Vatroslav Šerić ; Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia; Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia

Sažetak

Introduction: The aim was to evaluate the BD Barricor tubes by comparison with the BD Rapid Serum Tubes (RST) through measuring 25 analytes
and monitoring sample stability after 24 hours and 7 days.
Materials and methods: Samples of 52 patients from different hospital departments were examined. Blood was collected in BD RST and BD
Barricor tubes (Becton, Dickinson and Company, Franklin Lakes, USA). Analytes were measured by Beckman Coulter AU 480 (Beckman Coulter, Brea,
USA), Dimension EXL (Siemens Healthcare Diagnostics, Newark, USA) and ARCHITECT i2000SR (Abbott Diagnostics, Lake Forest, USA). Between-tube
comparison for each analyte was performed, along with testing analyte stability after storing samples at 4 °C.
Results: BD Barricor tubes showed unacceptable bias compared to BD RST tubes for potassium (K) (- 4.5%) and total protein (4.4%). Analyte stability
after 24 hours was acceptable in both tested tubes for most of analytes, except for glucose, aspartate aminotransferase (AST) and lactate dehydrogenase
(LD) in BD Barricor and free triiodothyronine in BD RST sample tubes. Analyte stability after 7 days was unacceptable for sodium, K, calcium,
creatine kinase isoenzyme MB, AST, LD and troponin I in both samples; additionally for glucose, alkaline phosphatase and albumin in BD Barricor.
Conclusion: All analytes, except K and total protein, can be measured interchangeably in BD RST and BD Barricor tubes, applying the same reference
intervals. For most of the analytes, sample re-analysis can be performed in both tubes after 24 hours and 7 days, although BD RST tubes show better
7-day analytes stability over BD Barricor tubes.

Ključne riječi

BD Barricor tube; stability; serum; plasma; evaluation

Hrčak ID:

244728

URI

https://hrcak.srce.hr/244728

Datum izdavanja:

15.10.2020.

Posjeta: 1.156 *