Uvodnik

Evaluation of Modified Yfiler™ Amplification Strategy for Compromised Samples

Kimberly A. Sturk ; Armed Forces DNA Identification Laboratory, Rockville, Md, USA
Michael D. Coble ; Armed Forces DNA Identification Laboratory, Rockville, Md, USA
Suzanne M. Barritt ; Armed Forces DNA Identification Laboratory, Rockville, Md, USA
Jodi A. Irwin ; Armed Forces DNA Identification Laboratory, Rockville, Md, USA

Sažetak

amplification protocol for the AmpFSTR® Yfiler™ PCR Amplification
Kit (Applied Biosystems) and explore the potential
of Y-chromosomal short tandem repeat (Y-STR) recovery
from severely degraded skeletal remains encountered
at the Armed Forces DNA Identification Laboratory.
Methods Experiments were performed using two sets of
Yfiler™ amplification parameters. One set of parameters
reflected the manufacturer’s recommendations. The second
set of parameters included twice the recommended
Taq concentration and 6 additional cycles. Recovery of authentic
alleles and the incidence of drop-in alleles were assessed
for 3 data sets: 8 different quantities of pristine DNA,
8 artificially-degraded samples, and 31 non-probative case
samples.
Results Samples tested with both protocols from all 3
data sets yielded twice as many authentic alleles under the
modified parameters than under the standard parameters
(62% vs 31%), with only a nominal associated increase in
the occurrence of non-authentic alleles (1.36% of all alleles
detected). When applied to a range of representative
casework samples, the modified protocol leveraged 9 or
more reproducible alleles from over half of the specimens
tested.
Conclusion Reproducible and informative Y-STR profiles
can be recovered from a broad range of degraded and
inhibited skeletal remains extracts when a commercially
available kit is employed under modified amplification parameters.

Ključne riječi

Y-chromosomal short tandem repeats (Y-STRs); degraded samples; low copy number

Hrčak ID:

40671

URI

https://hrcak.srce.hr/40671

Datum izdavanja:

15.6.2009.

Posjeta: 1.346 *