Veterinarski arhiv, Vol. 73 No. 4, 2003.
Izvorni znanstveni članak
Determination of P (F11) and F1 fimbriae of Escherichia coli isolated from avian cellulitis.
Reza Ghanbarpour
; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Amin Derakhshanfar
; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Ali Pourbakhsh
; Department of Poultry Science, Razi Vaccine and Serum Research Institute, Karaj, Iran
Sažetak
Avian cellulitis has been induced by virulent strains of E. coli. The purpose of this study was to determine P (F11) and F1 fimbriae of 90 E. coli isolates of avian cellulitis. Isolates were subjected to six consecutive passages on solid and static broth for expression of fimbriae. Five (5.5%) isolates from O1 serogroup, showed a mannose-resistant haemagglutination MRHA pattern when grown on solid medium. In the SDS-PAGE, crude fimbrial extracts of MRHA strain showed a major fimbrial subunit of 18 kDa. This band was also reacted with anti F11 serum on immunoblotting. Sixty-nine (76.6%) E. coli isolates from different serogroups showed mannose-sensitive hemagglutinating (MSHA) pattern when grown on static broth medium. In immunoblotting test, crude fimbrial extracts of MSHA isolates demonstrated a single band with 17 to 17.5 kDa apparent molecular weight as revealed by absorbed anti-F1A serum. It would appear that avian cellulitis E. coli isolates have F1 and P fimbriae similar to those of colisepticemic E. coli isolates.
Ključne riječi
cellulitis; chicken; broiler; E.coli; F1 fimbriae; P fimbriae
Hrčak ID:
74419
URI
Datum izdavanja:
20.8.2003.
Posjeta: 1.595 *