Veterinarski arhiv, Vol. 72 No. 6, 2002.
Izvorni znanstveni članak
Evaluation of RT-PCR for detection of Sudanese serotypes of epizootic hemorrhagic disease virus serogroup.
Mohammed Abdelsalam Abdalla
; Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Khartoum North, Sudan
Imadeldin Elamin Aradaib
; Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Khartoum North, Sudan
Bennie Irve Osburn
; Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California
Sažetak
Epizootic hemorrhagic disease (EHD) is an infectious non-contagious disease of deer and cattle. At least 2 serotypes of EHD virus, designated EHDV-4 and EHDV-318, are enzootic in the Sudan. To facilitate clinical disease investigation and control of the disease a rapid diagnostic assay is urgently needed. A reverse transcriptase (RT) polymerase chain reaction (RT-PCR) protocol, previously reported for detection of the United States EHDV serotypes 1 and 2 ribonucleic acid (RNA) in cell culture and clinical specimens, was evaluated for detection of the Sudanese EHDV serotypes. RNA from Sudanese isolates of EHDV-4 and EHDV-318, propagated in cell cultures, were detected by the described RT-PCR-based assay. The specific 387 bp PCR products were visualized on ethidium bromide stained agarose gel. Amplification product was not detected when the EHDV RT-PCR-based assay was applied to RNA from Sudanese bluetongue virus (BTV) serotype 4 (BTV-4) or total nucleic acid extracts from uninfected BHK-21 cells. The scientific observations reported in this paper indicated that the previously described EHDV RT-PCR assay could be applied for detection of EHDV infection among the Sudanese susceptible animal populations.
Ključne riječi
epizootic hemorrhagic disease; RT-PCR; Sudan
Hrčak ID:
81569
URI
Datum izdavanja:
18.12.2002.
Posjeta: 1.462 *