Izvorni znanstveni članak
PAX8-PPARg Oncogene in Follicular Thyroid Tumors: RT-PCR and Immunohistochemical Analyses
Biljana Pauzar
; »J. J. Strossmayer« University, Osijek University Hospital Center, Department of Clinical Cytology, Osijek, Croatia
Ivan Karner
; »J. J. Strossmayer« University, Osijek University Hospital Center, Clinical Institute of Nuclear Medicine and Radiation
Ljubica Glavaš-Obrovac
; »J. J. Strossmayer« University, Osijek University Hospital Center, Clinical Institute of Nuclear Medicine and Radiation
Mario Štefanić
; »J. J. Strossmayer« University, Osijek University Hospital Center, Clinical Institute of Nuclear Medicine and Radiation
Branko Dmitrović
; »J. J. Strossmayer« University, Osijek University Hospital Center, Department of Pathology and Forensic Medicine, Osijek,
Sažetak
US-guided fine needle aspiration cytology is currently the best diagnostic tool for thyroid nodules. However, it is not sensitive and specific enough for differentiating between benign and malignant follicular tumors. A potentially useful marker for this differentiation is the PAX8-PPARg rearrangement, identified in follicular thyroid carcinomas, but not in follicular adenomas or other types of thyroid tumors. The aim of this research was to determine the clinical significance of the PAX8-PPARg oncogene in diagnostics follicular thyroid tumors. The study included 62 patients with follicular or
Hürthle cell tumors. Gene expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) from paraffin embedded tissues, and PCR products were checked using the agarose gel electrophoresis. The immunohistochemical analysis was performed on archive paraffin embedded tissues with the monoclonal PPARã antibody. The statistical analysis has indicated that neither the expression of PAX8-PPARg mRNA, nor the immunohystochemical analysis with the PPARg antibody correlate with the patohystological diagnosis. The oncogene PAX8-PPARg has not met the expectations
as a reliable tumor marker for differentiation between benign and malignant thyroid tumors, which makes the only reliable histological criteria – capsular and vascular invasion.
Ključne riječi
follicular thyroid tumors; PAX8-PPARg rearrangement; RT-PCR; immunohistochemistry
Hrčak ID:
96460
URI
Datum izdavanja:
2.1.2013.
Posjeta: 1.439 *