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The Cytotoxic Effect of Sodium Hypochlorite on Cell Cultures

Ivana Miletić ; Zavod za bolesti zubi, Stomatološki fakultet Sveučilišta u Zagrebu, Gundulićeva 5 10000 Zagreb, Hrvatska
Alena Knežević ; Zavod za bolesti zubi, Stomatološki fakultet Sveučilišta u Zagrebu, Gundulićeva 5 10000 Zagreb, Hrvatska
Ivica Anić ; Zavod za bolesti zubi, Stomatološki fakultet Sveučilišta u Zagrebu, Gundulićeva 5 10000 Zagreb, Hrvatska
Maja Osmak ; Zavod za molekularnu genetiku, Institut "Ruđer Bošković", Bijenička cesta 54 10000 Zagreb, Hrvatska
Jozo Šutalo ; Zavod za bolesti zubi, Stomatološki fakultet Sveučilišta u Zagrebu, Gundulićeva 5 10000 Zagreb, Hrvatska
Zita Blažić-Potočki ; Stomatološka poliklinika, Perkovčeva 3, 10000 Zagreb, Hrvatska

Sažetak

Sodium hypochlorite (NaOCl) is the most popular irrigation solution used in root canal treatment, in spite of the fact that it may irritate the vital tissues. This study was designed to evaluate the cytotoxic effect of a water solution of sodium hypochlorite on cultures of human cervical carcinoma HeLa cells and mouse L929 fibroblasts by determining the number of vital cells. The tested concentrations of 0.5%, 1.0%, 2.5%, 5.25% were prepared in Eagle's growth medium. The number of cells was counted by electronic counter while the number of viable cells was determined, after the addition of nigrosin dye, by a light microscope. Concentration of 0.5% NaOCl caused the least changes in morphology, number and percentage of viable cells while 5.25% concentration caused total loss of HeLa and L929 cells. All tested concentrations were cytotoxic with significant difference (p<0.05) compared to the control group. However, the results showed that there was no significant difference between 1.0% and 2.5% concentration of NaOCl for both cell lines (p<0.05).

Ključne riječi

sodium hypochlorite; cell culture; cytotoxicity

Hrčak ID:

94757

URI

https://hrcak.srce.hr/94757

Datum izdavanja:

15.3.1999.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.591 *