Izvorni znanstveni članak

https://doi.org/10.11613/BM.2013.025

Diagnostic accuracy of UriSed automated urine microscopic sediment analyzer and dipstick parameters in predicting urine culture test results

Kağan Huysal ; Department of Clinical Laboratory, Yuksek Ihtisas Education and Research Hospital, Bursa, Turkey
Yasemin U. Budak ; Department of Clinical Laboratory, Sevket Yilmaz Education and Research Hospital, Bursa, Turkey
Ayse Ulusoy Karaca ; Department of Clinical Microbiology, Sevket Yilmaz Education and Research Hospital, Bursa, Turkey
Murat Aydos ; Department of Urology, Sevket Yilmaz Education and Research Hospital, Bursa, Turkey
Serdar Kahvecioğlu ; Department of Nephrology, Sevket Yilmaz Education and Research Hospital, Bursa, Turkey
Mehtap Bulut ; Department of Emergency Medicine, Sevket Yilmaz Education and Research Hospital, Bursa, Turkey
Murat Polat ; Department of General Surgery, Sevket Yilmaz Education and Research Hospital, Bursa, Turkey

Sažetak

Introduction: Urinary tract infection (UTI) is one of the most common types of infection. Currently, diagnosis is primarily based on microbiologic culture, which is time- and labor-consuming. The aim of this study was to assess the diagnostic accuracy of urinalysis results from UriSed (77 Electronica, Budapest, Hungary), an automated microscopic image-based sediment analyzer, in predicting positi-ve urine cultures.
Materials and methods: We examined a total of 384 urine specimens from hospitalized patients and outpatients attending our hospital on the same day for urinalysis, dipstick tests and semi-quantitative urine culture. The urinalysis results were compared with those of conventional semi-quantitative urine culture.
Results: Of 384 urinary specimens, 68 were positive for bacteriuria by culture, and were thus consi-dered true positives. Comparison of these results with those obtained from the UriSed analyzer indi-cated that the analyzer had a specificity of 91.1%, a sensitivity of 47.0%, a positive predictive value (PPV) of 53.3% (95% confidence interval (CI) = 40.8-65.3), and a negative predictive value (NPV) of 88.8% (95% Cl = 85.0-91.8%). The accuracy was 83.3% when the urine leukocyte parameter was used, 76.8% when bacteriuria analysis of urinary sediment was used, and 85.1% when the bacteriuria and leukocyturia parameters were combined. The presence of nitrite was the best indicator of culture positivity (99.3% specificity) but had a negative likelihood ratio of 0.7, indicating that it was not a relia-ble clinical test.
Conclusions: Although the specificity of the UriSed analyzer was within acceptable limits, the sensitivity value was low. Thus, UriSed urinalysis results do not accurately predict the outcome of culture.

Ključne riječi

urinalysis; bacteriuria; sensitivity; specificity; urinary tract infection

Hrčak ID:

103797

URI

https://hrcak.srce.hr/103797

Datum izdavanja:

15.6.2013.

Posjeta: 2.547 *