Izvorni znanstveni članak

Simultaneous detection and differentiation of porcine circovirus type 2, type 2 porcine reproductive and respiratory syndrome virus, porcine parvovirus and pseudorabies virus in pigs with PMWS by multiplex PCR.

Jian-Kui Liu ; College of Life Sciences, Longyan University, China; Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan University, China
Chun-Hua Wei ; College of Life Sciences, Longyan University, China; Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan University, China
Xiao-Yan Yang ; College of Life Sciences, Longyan University, China; Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan University, China
AI-Ling Dai ; College of Life Sciences, Longyan University, China; Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan University, China

Sažetak

A multiplex polymerase chain reaction (PCR) was designed for the simultaneous detection of four viruses involved in postweaning multisystemic wasting syndrome (PMWS) in pigs: porcine circovirus type 2 (PCV-2), type 2 porcine reproductive and respiratory syndrome virus (PRRSV), porcine parvovirus (PPV) and pseudorabies virus (PRV). Each of the four pairs of primers exclusively amplified the targeted fragment of the specific viruses. The sensitivity of the multiplex PCR, using purified plasmid constructs containing the specific viral target fragments, was 4.0×103, 4.5×103, 3.0×102 and 5.0×102 copies for PCV-2, type 2 PRRSV, PPV, PRV. Among 82 clinical samples, coinfection by PCV-2 and type 2 PRRSV was the most common. When compared with the virus isolation method commonly used to detect viruses, the multiplex PCR assay was found to be more sensitive and rapid and, as such, may prove to be a good alternative method for the detection of and differentiation in PMWS in pigs.

Ključne riječi

postweaning multisystemic wasting syndrome; porcine circovirus type 2; porcine reproductive and respiratory syndrome virus; porcine parvovirus; pseudorabies virus; PCR

Hrčak ID:

148012

URI

https://hrcak.srce.hr/148012

Datum izdavanja:

20.10.2015.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.845 *