Izvorni znanstveni članak

https://doi.org/10.11613/BM.2016.044

Urinary orosomucoid: validation of an automated immune turbidimetric test and its possible clinical use

Péter Kustán ; Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary
Balázs Szirmay ; Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary
Zoltán Horváth-Szalai ; Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary
Andrea Ludány ; Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary
Ágnes Lakatos ; Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary
Diána Mühl ; Department of Anaesthesiology and Intensive Therapy, University of Pécs Medical School, Pécs, Hungary
Per Hjort Christensen ; Dako A/S, Glostrup, Denmark
Attila Miseta ; Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary
Gábor L. Kovács ; Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary
Tamás Kőszegi ; Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary

Sažetak

Introduction: Besides routine serum markers of inflammatory diseases, the diagnostic potential of selected urinary proteins has not been fully exploited yet. Former studies revealed that urinary orosomucoid (u-ORM) might have complementary information in inflammatory disorders. Our aim was to develop and validate a fully automated method for u-ORM measurements and to evaluate its potential clinical impact on systemic inflammatory diseases.
Materials and methods: A particle-enhanced immune turbidimetric assay was validated for a Cobas 8000/c502 analyzer to determine u-ORM levels. Spot urine samples from 72 healthy individuals, 28 patients with Crohn’s disease and 30 septic patients were studied.
Results: Our assay time was 10 minutes and the detection limit of u-ORM was 0.02 mg/L. The intra- and inter-assay imprecision expressed as CV was less than 5%, and the recovery ranged between 95–103%. Within 10 to 60 years of age, a preliminary reference range for urinary orosomucoid/creatinine ratio (u-ORM/u-CREAT) was found to be 0.08 (0.01–0.24) mg/mmol [median (2.5–97.5 percentiles)]. Compared to controls, a five-fold increase of u-ORM/u-CREAT values in Crohn’s disease and approximately a 240-fold increase in sepsis were observed.
Conclusions: We set up a fast, sensitive and precise turbidimetric approach for automated u-ORM determination. Our highly sensitive assay is ideal for routine u-ORM measurements and might be a potential novel laboratory test in the management of systemic inflammatory processes.

Ključne riječi

orosomucoid; urine; particle-enhanced turbidimetry; inflammation; biomarker

Hrčak ID:

167731

URI

https://hrcak.srce.hr/167731

Datum izdavanja:

15.10.2016.

Posjeta: 1.448 *