Kratko priopćenje

https://doi.org/10.11613/BM.2018.020902

Comparison of Barricor™ vs. lithium heparin tubes for selected routine biochemical analytes and evaluation of post centrifugation stability

Anne Dupuy ; Department of Biochemistry, Lapeyronie University Hospital, Montpellier, France
Marie Stéphanie Badiou ; Department of Biochemistry, Lapeyronie University Hospital, Montpellier, France; PhyMedExp, INSERM U1046, CNRS UMR 9214, University of Montpellier, Montpellier, France
Delphine Daubin ; Intensive Care Medicine Department, Lapeyronie University Hospital, Montpellier, France
Anne Sophie Bargnoux ; Department of Biochemistry, Lapeyronie University Hospital, Montpellier, France; PhyMedExp, INSERM U1046, CNRS UMR 9214, University of Montpellier, Montpellier, France
Chloé Magnan ; Department of Biochemistry, Lapeyronie University Hospital, Montpellier, France
Kadda Klouche ; PhyMedExp, INSERM U1046, CNRS UMR 9214, University of Montpellier, Montpellier, France; Intensive Care Medicine Department, Lapeyronie University Hospital, Montpellier, France
Jean Paul Cristol ; Department of Biochemistry, Lapeyronie University Hospital, Montpellier, France; PhyMedExp, INSERM U1046, CNRS UMR 9214, University of Montpellier, Montpellier, France

Sažetak

Introduction: Obtaining suitable results unaffected by pre- or postanalytical phases is pivotal for clinical chemistry service. We aimed comparison
and stability of nine biochemical analytes after centrifugation using Barricor™ plasma tubes with mechanical separator vs standard Vacutainer®
lithium heparin tubes.
Materials and methods: We collected samples on six healthy volunteers and nine patients from intensive care units into 6 mL plastic Vacutainer®
lithium heparin tubes and 5.5 mL plastic Barricor™ plasma tubes. All tubes were centrifuged within 30 minutes after venipuncture. First, we compared
results of nine biochemical analytes from lithium heparin tubes with Barricor™ tubes for each analyte using Passing-Bablok and Bland-Altman
analyses. Second, we calculated the difference of analyte concentrations between baseline and time intervals in tubes stored at + 4 °C. Based on the
total change limit we calculated the maximum allowable concentrations percentage change from baseline.
Results: The majority of correlation coefficients were close to 0.99 indicating good correlation in the working range. Bland-Altman analyses showed
an acceptable concordance for all analytes. In consequence, the Barricor™ tube might be an alternative to regular lithium heparin tube. Stability with
this new generation tube is improved for eight analytes (except for aspartate aminotransferase) in comparison with regular lithium heparin tubes.
Conclusions: By using Barricor™ tubes and prompt centrifugation, supplemental analysis or re-analysis for eight analytes including alanine aminotransferase,
alkaline phosphatase, C-reactive protein, high sensitivity troponin T, lactate dehydrogenase, NT-pro BNP, potassium and sodium could
be performed within 72 h of specimen collection.

Ključne riječi

blood specimen collection; plasma; test tube; evaluation

Hrčak ID:

199001

URI

https://hrcak.srce.hr/199001

Datum izdavanja:

15.6.2018.

Posjeta: 1.371 *