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URINE CONCENTRATIONS OF HIGH-SENSITIVITY CARDIAC TROPONIN I IN HEALTHY ADULTS – PRELIMINARY REFERENCE INTERVALS
PETRA PERVAN
; Dom zdravlja Zagreb-Centar, Zagreb, Hrvatska
TOMO SVAGUŠA
orcid.org/0000-0002-2036-1239
; Medicinski fakultet Sveučilišta u Zagrebu, Klinička bolnica Dubrava, Klinika za unutarnje bolesti, Zagreb, Hrvatska
INGRID PRKAČIN
orcid.org/0000-0002-5830-7131
; Medicinski fakultet Sveučilišta u Zagrebu, Klinička bolnica Merkur, Klinika za unutarnje bolesti, Zagreb, Hrvatska
JURICA VUKOVIĆ
; Klinička bolnica Merkur, Odjel za urologiju, Zagreb, Hrvatska
ANDREA RADELJAK
; Klinička bolnica Merkur, Klinički zavod za medicinsku biokemiju i laboratorijsku medicinu, Referentni centar Ministarstva zdravstva Republike Hrvatske za izradu referentnih vrijednosti u području opće medicinske biokemije, Zagreb
SONJA PERKOV
; Klinička bolnica Merkur, Klinički zavod za medicinsku biokemiju i laboratorijsku medicinu, Referentni centar Ministarstva zdravstva Republike Hrvatske za izradu referentnih vrijednosti u području opće medicinske biokemije, Zagreb
Sažetak
Recent studies have shown the presence of troponin molecules in urine, with kidneys considered the main organs of elimination. Availability of the new generation of high-sensitivity assays has enabled detection of low concentrations of circulating cardiac troponins, but the high-sensitivity troponin assays are not designed for analysis of urine specimens. The aim of this study was to establish preliminary reference intervals for the high-sensitivity cardiac troponin I (hs-cTnI) concentrations in urine of healthy adults in Croatia. A total of 60 reference persons were selected (30 males and 30 females) and the concentrations of hs-cTnI in random urine samples were determined on the Abbott Architect i1000SR (Abbott Laboratories) analytical system with chemiluminescent immunochemical method on microparticles (CMIA, ARCHITECT STAT High Sensitive Troponin-I), accredited according to the HR EN ISO 15189:2012 standard (Medical laboratorieds – requirements for quality and competence. Geneva, International Organization for Standardization, 2012). We determined the limit of detection, total laboratory precision, expanded measurement uncertainty and preliminary estimates of the gender-specifi c 99th percentile of the upper reference limit (URL) using nonparametric analysis (methods). The male and female 99th percentile cut-off values were 39.3 and 35.2 pg/mL, respectively. The results of this pilot study suggest that troponin I is removed from the blood by the kidneys and can be determined in the urine with CMIA, ARCHITECT STAT High Sensitive Troponin-I assays. Further research is focused on detailed studies of biochemistry and determination of troponin I in the urine as a new biological marker.
Ključne riječi
troponin I; determination in urine
Hrčak ID:
216412
URI
Datum izdavanja:
30.1.2019.
Posjeta: 1.933 *