Veterinarska stanica, Vol. 52 No. 3, 2021.
Izvorni znanstveni članak
https://doi.org/10.46419/vs.52.3.6
Microsatellite markerbased genetic diversity in Mareecha and Barela breeds of dromedary camel from Pakistan
Tanveer Hussain
; Department of Molecular Biology, Virtual University of Pakistan
Fiaz Hussain
; School Education Department, Dera Ghazi Khan, Punjab
Abdul Wajid
; Department of Biotechnology, Virtual University of Pakistan, Pakistan
Masroor Ellahi Babar
; The University of Agriculture, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan
Muneeb M. Mustafa
; Department of Biosystems Technology, Faculty of Technology, South Eastern University of Sri Lanka, University Park, Oluvil, #32360, Sri Lanka
Faiz M. M. T. Marikar
orcid.org/0000-0003-4579-7263
; General Sir John Kotelawala Defense University, Ratmalana, Sri Lanka
Sažetak
The genetic diversity of Pakistani dromedary camels is poorly documented. The present study evaluated the genetic variations of two well-known Pakistani camel breeds, Mareecha and Barela, that are well-adapted to the Cholistan desert climate. Camel can serve
as a beneficial participant in the food supply chain by providing milk, meat and other food products for the livelihood of pastoral peoples. To explore a new world of resources, greater attention to needed to create standard procedures to genetically characterize, classify and identify camel breeds in the country. For this purpose, 66 unrelated animals of the Mareecha (n=35) and Barela (n=31) breeds were genotyped using a set of 12 labelled microsatellite loci. DNA fragment sizes were determined in an ABI 3130 Genetic Analyzer. All microsatellite markers were successfully amplified and exhibited a polymorphic nature, with an average Polymorphic Information Content (PIC) of 0.72 and 0.70 in Mareecha and Barela, respectively. A total of 107 alleles with an average of 8.91 alleles per locus were identified by these markers in both breeds. CMS15 was highly polymorphic with 13 alleles, while VOLP-032 was the lowest with two loci. The Fit and Fis values were low but high population differentiation (17%) was observed in both breeds, due to the migrations of pastoral people to different remote areas during climate fluctuations.
Ključne riječi
pasmine deva; genetska raznolikost; mikrosatelit; Pakistan
Hrčak ID:
247370
URI
Datum izdavanja:
16.12.2021.
Posjeta: 2.210 *