Medicina, Vol. 58 No. 3, 2022.
Izvorni znanstveni članak
https://doi.org/10.21860/medflum2022_281001
Development and validation of the GC-MS method for determining the influence of extraorporeal membrane oxygenation (ECMO) devices on changes in plasma rocuronium bromide concentration
Anamarija Smirčić
; Dom zdravlja Primorsko-goranske županije, Rijeka, Hrvatska
Tanja Lovrinov
orcid.org/0000-0001-8680-4883
; Specijalna bolnica Medico, Rijeka, Hrvatska
Željko Jovanović
orcid.org/0000-0003-0476-9303
; Sveučilište Josipa Jurja Strossmayera u Osijeku, Medicinski fakultet, Osijek, Hrvatska, Sveučilište u Rijeci, Fakultet zdravstvenih studija, Katedra za temeljne medicinske znanosti, Rijeka, Hrvatska
Sažetak
Objective: Gas chromatography with mass spectrometry (GC-MS) is a sensitive and selective method with the potential for standard determination of rocuronium bromide in plasma. The use of Extracorporeal Membrane Oxygenation (ECMO) can be used to determine the effect on drug concentrations. The aim of the study was to examine the suitability of the GC-MS method for the determination of rocuronium bromide in plasma and to compare its venous and arterial concentrations in patients with ECMO support. Materials and methods: Standard solutions of different ratios, plasma and rocuronium bromide solution (Esmeron, 10 mg/ml, N.V. Organon, The Netherlands), ethanol, dichloromethane, sodium dihydrogen phosphate, potassium iodide, water, acetone were used. GC-MS was performed in the Teaching Institute for Public Health Rijeka, and the clinical part on five randomly selected patients treated with ECMO support in University hospital of Rijeka. Results: obtained by validation are: GD 0.553 μg/ml, GK (confirmed) 5 μg/ml, accuracy RSD = 2.083% (RSD ≤ 10%), intermediate accuracy RSD = 3.05% (RSD ≤ 10%), accuracy 98.9% (90 – 110%), correlation coefficient k = 0.9961 (r ≥ 0.995), selectivity of the method – RT = 23.25 min, m/z = 413. The clinical value of the method was confirmed by measuring the concentrations of rocuronium bromide in patients with ECMO support. Rocuronium bromide values were higher in the venous than in the arterial sample in all measurements. Conclusions: Validation of the GC-MS method confirmed that all performed validation parameters are in the acceptance criterion and the method is applicable for the determination of rocuronium bromide. ECMO support reduces the drug level in the arterial sample, but due to the small number of samples and high variability in concentration, no statistically significant difference was achieved.
Ključne riječi
chromatography, gas; extracorporeal membrane oxygenation; rocuronium
Hrčak ID:
281001
URI
Datum izdavanja:
1.9.2022.
Posjeta: 1.373 *