Izvorni znanstveni članak
Glutathione S-transferase and Microsomal Epoxide Hydrolase Gene Polymorphisms and Risk of Chronic Obstructive Pulmonary Disease in Slovak Population
Jozef Židzik
; Zavod za medicinsku biologiju Medicinskog fakulteta, Košice, Slovačka
Eva Slabá
; Zavod za medicinsku biologiju Medicinskog fakulteta, Košice, Slovačka
Pavol Joppa
; Zavod za respiratorne bolesti i tuberkulozu, Medicinski fakultet Sveučilišta, Košice, Slovačka
Zuzana Kluchová
; Zavod za respiratorne bolesti i tuberkulozu, Medicinski fakultet Sveučilišta, Košice, Slovačka
Zuzana Dorková
; Zavod za respiratorne bolesti i tuberkulozu, Medicinski fakultet Sveučilišta, Košice, Slovačka
Peter Skyba
; Zavod za respiratorne bolesti i tuberkulozu, Medicinski fakultet Sveučilišta, Košice, Slovačka
Viera Habalová
; Zavod za medicinsku biologiju Medicinskog fakulteta, Košice, Slovačka
Ján Šalagovič
; Zavod za medicinsku biologiju Medicinskog fakulteta, Košice, Slovačka
Ružena Tkáčová
; Zavod za respiratorne bolesti i tuberkulozu, Medicinski fakultet Sveučilišta, Košice, Slovačka
Sažetak
Aim To determine the risk of chronic obstructive pulmonary disease
(COPD) associated with polymorphisms in the glutathione S-transferase
(GST) M1, GST T1, and microsomal epoxide hydrolase (EPHX1)
genes in a cohort of Slovak population.
Methods Two hundred and seventeen patients with the diagnosis of
COPD and 160 control subjects were enrolled in the study. Blood
samples were collected from all subjects and the DNA from peripheral
blood lymphocytes was used for subsequent genotyping assays, using
polymerase chain reaction and restriction fragment-length polymorphism
methods.
Results In an unadjusted model, an increased risk for COPD was observed
in subjects with EPHX1 His113-His113 genotype (odds ratio
[OR], 2.32; 95% confidence interval [CI], 1.20-4.69; P = 0.008), compared
with the carriers of the Tyr113 allele. However, after the adjustments
for age, sex, and smoking status, the risk was not significant (adjusted
OR, 1.79; 95% CI, 0.91-3.53; P = 0.093). In a combined analysis
of gene polymorphisms, the genotype combination EPHX1 His113-
His113/GSTM1 null significantly increased the risk of COPD in both,
unadjusted (OR, 5.08; 95% CI, 1.70-20.43; P = 0.001) and adjusted
model (OR, 4.87; 95% CI, 1.57-15.13; P = 0.006).
Conclusion Although none of the tested gene polymorphisms was significantly
related to an increased risk of COPD alone, our results suggest
that the homozygous exon 3 mutant variant of EPHX1 gene in the
combination with GSTM1 null genotype is a significant predictor of
increased susceptibility to COPD in the Slovak population. The findings
of the present study emphasize the importance of detoxifying and
antioxidant pathways in the pathogenesis of COPD.
Ključne riječi
Chronic obstructive pulmonary disease; Glutathione S-transferase; Microsomal epoxide hydrolase, Gene polymorphism
Hrčak ID:
26108
URI
Datum izdavanja:
15.4.2008.
Posjeta: 2.062 *