Izvorni znanstveni članak
https://doi.org/10.2478/cjf-2022-0018
EXPLORING FISH MISCELLANY FROM BIODIVERSITY HOTSPOT REGION OF NORTHEAST INDIA THROUGH CHROMOSOME PROFILING
Basdeo Kushwaha
; ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow-226 002, India
Dandadhar Sarma
; Department of Zoology, Gauhati University, Guwahati – 781014, Assam, India
Banasri Mech
; Department of Zoology, Gauhati University, Guwahati – 781014, Assam, India
Murali S Kumar
; ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow-226 002, India
Vishwamitra Singh Baisvar
; ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow-226 002, India
Avinash Tiwari
; ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow-226 002, India
Hrishikesh Choudhury
; Department of Zoology, Gauhati University, Guwahati – 781014, Assam, India
Ravindra Kumar
; ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow-226 002, India
Sažetak
North Eastern states of India are blessed with extensive ecoclimatic conditions and much of India’s endemic flora and fauna. This region is rich in fish diversity with plenty of species of ornamental importance, including model species zebrafish. Among the potential ornamental fishes, Ctenops nobilis is designated as Near Threatened and Danio dangila is included in the Least Concern list by IUCN. Cytogenetic profiling was undertaken here to provide complementary data for precise species identification and to study genetic inheritance, genome organization and evolution of the species. The cytogenetic analyses revealed diploid chromosome numbers 44 and 50 with karyotype formula 8m+20sm+10st+6t (FN=72) and 20m+24sm+6st (FN=94), respectively, in C. nobilis and D. dangila. The staining of nucleolar organizer regions revealed the presence of Ag-NORs, CMA3 sites and 18S rDNA probe signals on one pair sub-telocentric chromosome and one pair sub-metacentric chromosome in C. nobilis and D. dangila, respectively. These biomarkers are an important resource for molecular taxonomy, evolutionary/phylogenetic studies and conservation genetics of C. nobilis and D. dangila.
Ključne riječi
18S rDNA; Ag-NOR; Ctenops nobilis; Danio dangila; FISH; Karyotype
Hrčak ID:
287198
URI
Datum izdavanja:
14.12.2022.
Posjeta: 310 *