Izvorni znanstveni članak

Effect of intensive insulin therapy on systemic nitric oxide levels and adenosine deaminase activity in secondary sulfonylurea failure

Ayje Binnur Erbağci ; Department of Biochemistry and Clinical Biochemistry, School of Medicine, University of Gaziantep, Gaziantep, Turkey
Rukiye Deveci ; Department of Biochemistry and Clinical Biochemistry, School of Medicine, University of Gaziantep, Gaziantep, Turkey
Ersin Akarsu ; Internal Medicine, School of Medicine, University of Gaziantep, Gaziantep, Turkey
Özlem Tiryaki ; Internal Medicine, School of Medicine, University of Gaziantep, Gaziantep, Turkey
Nur Aksoy ; Department of Biochemistry and Clinical Biochemistry, School of Medicine, University of Gaziantep, Gaziantep, Turkey

Sažetak

Background: Inflammation, oxidative stress and apoptosis are suggested to take part in secondary sulfonylurea failure associated with β cell destruction and impaired β cell function. Nitric oxide (NO) stimulates insulin release, has proinflammatory, apoptotic and free radical effects. Adenosine deaminase (ADA) exerts control on adenosine levels, which affects insulin and glucagon release and peripheral glucose metabolism. In this study, we aimed to investigate ADA activity and NO levels as potential mediators of intensive insulin treatment in patients with secondary sulfonylurea failure.
Materials and methods: Twenty-four patients with type 2 diabetes mellitus and secondary sulfonylurea failure were enrolled in the study. Serum NO levels and ADA activity were determined in blood samples obtained prior to treatment, after three-day insulin infusion and after six-month multiple subcutaneous insulin administration. ADA activities were estimated by the method of Giusti. Nitric oxide levels were measured with colorimetric assay by the method of Griess reaction.
Results: In secondary sulfonylurea failure, baseline ADA activity (17.0 [14.6-21.7] U/L) was significantly lower than ADA activity measured on day 3 (20.5 [16.2-23.4] U/L; P = 0.018) and at 6 months (21.2 [16.6-22.5] U/L; P = 0.010). There was no statistically significant difference between NO levels determined before (18.8 [11.6-28.4] μmol/L), on day 3 (17.8 [9.7-33.6] μmol/L) and at 6 months (21.7 [16.0-33.9] μmol/L; P = 0.966 and P = 0.230, respectively).
Conclusions: According to our study, ADA activity is increased both in the early and late periods of intensive insulin therapy in secondary sulfonylurea failure. The role of NO in amelioration of β cell function is not supported at the level of peripheral blood.

Ključne riječi

adenosine deaminase; intensive insulin therapy; nitric oxide; secondary sulfonylurea failure

Hrčak ID:

37618

URI

https://hrcak.srce.hr/37618

Datum izdavanja:

3.6.2009.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.921 *